GRF1 Antibody

GRF1 Antibody Overview

GRF1 antibodies target Ras-GRF1, a 155 kDa guanine nucleotide exchange factor (GEF) that activates Ras and Rac GTPases by catalyzing GDP-to-GTP exchange . Ras-GRF1 contains distinct domains:

• CDC25 homology domain (Ras-GEF activity)

• IQ motif (calmodulin binding)

• Dbl homology (DH) and pleckstrin homology (PH) domains (Rac-GEF activity)

These antibodies are essential for detecting GRF1 expression in tissues like pancreatic islets, brain, and testis .

Pancreatic β-Cell Development

GRF1 antibodies revealed reduced β-cell mass in GRF1 knockout mice due to impaired proliferation and neogenesis :

• 30% reduction in serum insulin levels

• Defective IGF-I signaling: Akt and Erk activation failure in β-cells

• Normal insulin secretion but reduced β-cell area (Figure 6B)

Neuronal Signaling

GRF1 regulates synaptic plasticity via:

• Ras-ERK pathways: Essential for long-term memory

• Rac-JNK1 activation: Mediated by Gβγ subunits (Figure 3A)

  • Synergistic JNK1 activation with Gβγ requires intact DH domain

Metabolic Regulation

GRF1-deficient mice exhibit:

• Increased lipid catabolism: 30% lower gonadal fat mass

• Glucose intolerance: Delayed insulin secretion during glucose challenge

Mechanistic Insights from Antibody-Based Studies

Protocols and Validation

• Proteintech’s RASGRF1 antibody (12958-1-AP) validates in WB (1:500–1:2000 dilution), IHC (1:50–1:200), and IP .

• Cell Signaling’s #3322 detects transfected GRF1 in mouse models .

Implications for Disease Research

• Diabetes: GRF1’s role in β-cell expansion highlights therapeutic potential for insulin deficiency .

• Neurodegeneration: Altered GRF1/ERK signaling correlates with memory deficits .

• Cancer: Unregulated Ras-GRF1 activity may drive oncogenic Ras signaling.