pptr-1 Antibody

Introduction to PPTR-1 Antibody

PPTR-1 is a regulatory subunit of the PP2A holoenzyme in C. elegans, involved in dephosphorylation events that modulate muscle development and RNA granule dynamics . The antibody targeting PPTR-1 enables precise localization and functional studies of this protein.

Key Attributes

Recommended Dilutions

Sarcomere Organization

PPTR-1 localizes to I-bands in C. elegans muscle cells, as demonstrated by immunofluorescence . Loss of PPTR-1 disrupts sarcomere structure, leading to:

• Misalignment of actin filaments

• Abnormal muscle contraction patterns

RNA Granule Dynamics

PPTR-1 regulates P granule stability during embryonic development:

• Acts redundantly with PPTR-2 to promote granule assembly

• Depletion causes premature granule disassembly during mitosis

Functional Interactions

• Genetic Interaction: Works downstream of daf-2 insulin/IGF-1 receptor to modulate lifespan and stress resistance .

• Biochemical Role: Regulates AKT-1 phosphorylation at Thr 350, a conserved mechanism shared with mammalian B56β .

Western Blot Analysis

• Observed Band: 32–34 kDa (matches predicted molecular weight)

• Knockout Control: No detectable signal in pptr-1(tm3103) mutants

Localization Studies

• I-Band Specificity: Anti-PPTR-1 immunofluorescence highlights I-bands, distinct from PPTR-2’s M-line localization .

Comparative Insights

Technical Considerations

• Storage: Stable at -20°C for one year; avoid repeated freeze-thaw cycles .

• Cross-Reactivity: No cross-reactivity with PPTR-2 due to unique C-terminal epitopes .