4CL1 Antibody
Description
Target Overview: 4CL1 Enzyme
4CL1 (UniProt ID: Q8S564) is a 546-amino-acid enzyme critical for synthesizing phenylpropanoid-derived compounds, including lignin, flavonoids, and suberin . It catalyzes the formation of CoA thioesters from hydroxycinnamic acids (e.g., 4-coumarate, caffeate), which serve as precursors for diverse plant metabolites .
Key Functions
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Lignin Biosynthesis: 4CL1 is indispensable for lignin deposition in vascular plants. Knockout mutants of 4CL1 in Populus and Arabidopsis show 19–34% reductions in lignin content and altered lignin monomer ratios (syringyl/guaiacyl) .
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Metabolic Regulation: Compensatory shifts in phenylpropanoid flux occur in 4cl1 mutants, including increased p-coumarate and caffeate accumulation .
Lignin Modification in Populus
Cell-specific suppression of 4CL1 in Populus fibers and vessels reduced lignin content by 19% without affecting growth . Key changes included:
| Parameter | Control | 4cl1 Mutant | Change | P Value |
|---|---|---|---|---|
| Total Lignin (%) | 15.94 ± 0.40 | 12.93 ± 0.37 | −19% | <0.001 |
| S/G Lignin Ratio | 1.81 ± 0.02 | 1.26 ± 0.01 | −30% | <0.001 |
| H-Lignin (µmol/g) | 29.15 ± 1.01 | 44.19 ± 1.20 | +52% | <0.001 |
This reduction coincided with compensatory increases in pectin-associated sugars (e.g., glucuronic acid +16%) and altered lignin-carbohydrate cross-linking .
Arabidopsis Studies
In Arabidopsis, 4CL1 knockout mutants retained only 10% of total 4CL activity, underscoring its dominance in lignin biosynthesis . Higher-order mutants (e.g., 4cl1 4cl2 4cl3) exhibited severe lignin defects, with guaiacyl lignin dropping to 50 mol% .
Applications in Plant Biotechnology
The 4CL1 antibody enables:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
