4CLL9 Antibody

Overview of 4CLL9 Antibody

The 4CLL9 antibody is a mouse-derived monoclonal antibody targeting the 4-coumarate-CoA ligase-like 9 (4CLL9) protein in Arabidopsis thaliana. This antibody is primarily utilized in plant biology research to study phenylpropanoid metabolic pathways, which are critical for lignin biosynthesis, flavonoid production, and stress responses .

Role in Plant Stress Responses

4CLL9 is a member of the 4-coumarate-CoA ligase-like (4CL) family, enzymes involved in the phenylpropanoid pathway. Studies using the 4CLL9 antibody revealed its upregulation in transgenic Citrus aurantifolia plants engineered to express the PRpnp gene, which enhances abiotic stress tolerance . Key findings include:

This suggests 4CLL9 contributes to stress adaptation by regulating secondary metabolites linked to pathogen defense and drought resistance .

Subcellular Localization

Immunoassays localize 4CLL9 to peroxisomes, organelles involved in lipid metabolism and reactive oxygen species detoxification. This aligns with its role in metabolizing phenolic compounds during oxidative stress .

Applications in Plant Research

• Western Blot: Validates 4CLL9 expression in Arabidopsis and rice lysates .

• ELISA: Quantifies protein levels in transgenic plant models (recommended dilution: 1:1,000–1:3,000) .

• Functional Studies: Links 4CLL9 activity to abscisic acid (ABA)-mediated stress pathways and jasmonate synthesis .