Customize AAE17 Antibody

Description

Definition and Target Identification

The AAE17 antibody (Product Code: CSB-PA520719XA01DOA) is a polyclonal antibody raised against the AAE17 protein in Arabidopsis thaliana . The AAE17 protein is encoded by the UniProt accession F4KBF3, though its specific biological role remains underexplored in publicly available literature.

Key Attributes:

Property	Detail
Target Species	Arabidopsis thaliana (Mouse-ear cress)
Antibody Type	Polyclonal
Host Species	Not specified (typically rabbit or mouse)
Applications	Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Available Sizes	2 mL (working solution), 0.1 mL (purified)

Experimental Use Cases

AAE17 antibodies are likely employed in:

Protein Localization: Tracking AAE17 expression in plant tissues via IHC/IF .
Functional Knockdown Studies: Validating AAE17 protein depletion in genetic mutants.

Unresolved Questions

No peer-reviewed studies directly addressing AAE17’s role in Arabidopsis were identified in the provided sources.
The UniProt entry (F4KBF3) lacks detailed functional annotations, suggesting AAE17 is either novel or understudied.

Comparative Analysis with Related Antibodies

The Cusabio database lists over 50 antibodies targeting Arabidopsis proteins, including AAE17 . A subset is compared below:

Antibody Name	Target Protein	UniProt ID	Applications
AAE17	AAE17	F4KBF3	WB, IHC, IF
ABCB25	ABCB25	Q9LVM1	ELISA, WB
AGP31	AGP31	Q9FZA2	IHC, Immunoprecipitation

Future Directions

Structural Resolution: Techniques like X-ray crystallography or cryo-EM could map AAE17’s epitope binding sites, leveraging databases like AACDB .
Functional Studies: Linking AAE17 to specific pathways (e.g., abiotic stress responses) using knockout plant models.

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

AAE17 antibody; At5g23050 antibody; MYJ24.4Probable acyl-activating enzyme 17 antibody; peroxisomal antibody; EC 6.2.1.- antibody

Target Names

AAE17

Uniprot No.

F4KBF3

Target Background

Function

AAE17 Antibody may function as an acid-thiol ligase, activating carboxylic acids by forming acyl-CoAs.

Database Links

KEGG: ath:AT5G23050

STRING: 3702.AT5G23050.1

UniGene: At.43307

Protein Families

ATP-dependent AMP-binding enzyme family

Subcellular Location

Peroxisome.

Tissue Specificity

Expressed in leaves, stems and developing seeds.

Q&A

AAE17 Antibody Research FAQs

Advanced Research Challenges

How to resolve discrepancies in AAE17 neutralization data between in vitro and in vivo models?

Troubleshooting Framework:
- Pharmacokinetic Factors: Measure antibody half-life and tissue penetration using radiolabeled tracers .
- Target Saturation: Perform dose-response curves with occupancy assays (e.g., PET imaging) .
- Species-Specific Activity: Validate cross-reactivity in model organisms via epitope mapping (Table 2) .

What strategies enable structural analysis of AAE17-antibody complexes?

Table 2: Structural Biology Approaches

Technique	Resolution (Å)	Sample Requirement	Key Application
X-ray Crystallography	1.5-3.0	High-purity complex	Atomic epitope mapping
Cryo-EM	2.5-4.0	Heterogeneous samples	Conformational dynamics
HDX-MS	N/A	Solution state	Binding interface thermodynamics

How to engineer AAE17 antibodies for enhanced stability while retaining neutralization potency?

Rational Design Protocol:
- Framework Optimization: Transplant CDRs onto stable human germline scaffolds (e.g., VH3-23/VK1-39) .
- Aggregation Resistance: Introduce surface mutations (e.g., Gln→Glu at position 82) using structure-guided design .
- In Silico Screening: Perform molecular dynamics simulations (≥100 ns) to predict thermal stability .

Data Interpretation Guidelines

How to distinguish direct vs. indirect effects in AAE17 functional studies?

Experimental Triangulation:
- Combine antibody treatment with:
  - RNA-seq to identify primary transcriptional targets
  - Phospho-proteomics to map downstream signaling nodes
  - CRISPR knockout of putative intermediate effectors

What statistical methods address high variability in AAE17 immunohistochemical data?

Robust Analysis Pipeline:
- Normalization: Use reference antibodies against housekeeping proteins (e.g., GAPDH) .
- Quantification: Apply machine learning-based image analysis (e.g., QuPath for H-score calculation) .
- Batch Correction: Include inter-assay controls and apply ComBat normalization .

Translational Considerations

How to validate AAE17 antibody specificity across evolutionary distant species?

Phylogenetic Coverage Strategy:
- Test binding against:
  - Primate orthologs (≥85% sequence identity)
  - Rodent variants (site-directed mutants for human epitopes)
  - Artificial chimeric proteins with swapped domains

What in vivo models best recapitulate AAE17 antibody pharmacodynamics?

Model Selection Criteria:

Model Type	Human Target Expression	Immune Competence	Key Application
Humanized IL17R mice	Endogenous	Partial	Efficacy/safety profiling
Non-human primates	Cross-reactive	Full	Preclinical PK/PD
3D organoid systems	Tissue-specific	N/A	Mechanistic studies

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AAE17 Antibody