Customize AAPT1 Antibody

Description

Introduction to AAPT1 Antibody

AAPT1 (CHPT1) is a 406-amino-acid multi-pass membrane protein localized to the Golgi apparatus. It catalyzes the final step of phosphatidylcholine synthesis by transferring choline phosphate from CDP-choline to diacylglycerol (DAG), critical for vesicular membrane formation and maintenance . The AAPT1 antibody is a polyclonal rabbit-derived reagent used to study CHPT1 in research settings .

Antibody Characteristics

Key properties of the AAPT1 antibody include:

Property	Detail
Target	CHPT1 (UniProt ID: Q8WUD6; Entrez Gene ID: 56994)
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Applications	Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Immunogen	KLH-conjugated synthetic peptide (human CHPT1, AA 7-33)
Purification	Protein A

Biological Role

CHPT1 is highly expressed in testis, small intestine, heart, and cancerous breast cells .
It is upregulated in breast cancer cell lines compared to normal tissues, suggesting a role in oncogenesis .
Mustard gas exposure downregulates CHPT1, potentially contributing to acute respiratory distress syndrome (ARDS) .

Clinical Implications

Study Focus	Key Observation
Cancer Biology	Increased CHPT1 expression correlates with tumor progression .
Environmental Toxicity	CHPT1 suppression by mustard gas links to ARDS pathogenesis .
Therapeutic Targets	CHPT1’s role in membrane synthesis highlights its potential in drug development .

Data Validation

Western Blot: AAPT1 antibody detects a ~37 kDa band in human, mouse, and rat lysates .
Immunofluorescence: Nuclear localization confirmed in HCT116 (colorectal carcinoma) and NIH/3T3 (fibroblast) cells .
Flow Cytometry: Specific binding observed in permeabilized NIH/3T3 cells .

Comparative Analysis

Feature	AAPT1 Antibody (ab189474)	CHPT1 Antibody (PA5-23695)
Host Species	Rabbit	Rabbit
Applications	WB, IF, IHC	WB, Flow Cytometry
Immunogen Region	AA 7-33 (human)	Full-length protein (UniProt Q8WUD6)

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 week lead time (made-to-order)

Synonyms

AAPT1 antibody; At1g13560 antibody; F13B4.5 antibody; Choline/ethanolaminephosphotransferase 1 antibody; EC 2.7.8.1 antibody; EC 2.7.8.2 antibody; Aminoalcohol phosphotransferase 1 antibody; AtAAPT1 antibody

Target Names

AAPT1

Uniprot No.

O82567

Target Background

Function

This antibody targets AAPT1, an enzyme that catalyzes the biosynthesis of both phosphatidylcholine and phosphatidylethanolamine from CDP-choline and CDP-ethanolamine, respectively. It exhibits greater cholinephosphotransferase activity than ethanolaminephosphotransferase activity.

Database Links

KEGG: ath:AT1G13560

STRING: 3702.AT1G13560.1

UniGene: At.14784

Protein Families

CDP-alcohol phosphatidyltransferase class-I family

Subcellular Location

Membrane; Multi-pass membrane protein.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

AAPT1 Antibody