At3g28510 Antibody

Molecular Characterization of AT3G28510

The AT3G28510 gene encodes a P-loop containing nucleoside triphosphate hydrolase, part of the AAA+ ATPase superfamily . This enzyme is implicated in energy-dependent processes such as protein degradation, DNA repair, and stress response modulation.

Key Features of the AT3G28510 Protein:

• Domain Structure: Contains conserved ATPase motifs critical for substrate binding and hydrolysis .

• Function: Likely acts as a negative regulator of stress-related genes, with homologs in tobacco (NtAAA1) shown to attenuate hypersensitive responses to pathogens .

Role in Plant Stress Responses

• Upregulation During Viral Infection: The A. thaliana homolog of AT3G28510 (AAA-type ATPase) is upregulated during infections by Tomato yellow leaf curl Sardinia virus (TYLCSV) and other RNA viruses . This suggests involvement in modulating cellular stress pathways.

• Transcriptional Alterations: Transgenic tomato plants expressing Rep130 (a viral replication protein) show altered transcriptional profiles, including upregulation of AT3G28510-related genes. This correlates with enhanced resistance to TYLCSV .

Functional Insights from Homologs

• Tobacco Homolog (NtAAA1): Attenuates hypersensitive responses to pathogens, preventing excessive cell death .

• Arabidopsis Homolog (AATP1): Proposed to downregulate stress-related genes, balancing defense and growth .

Applications in Plant Biology

At3g28510 Antibody is utilized to:

1. Track Protein Expression: Monitor AAA+ ATPase levels under biotic/abiotic stress conditions.

2. Study Pathogen Interactions: Investigate how viral proteins (e.g., TYLCSV Rep130) hijack host ATPases for replication .

3. Elucidate Defense Mechanisms: Characterize the role of ATPases in suppressing immune overactivation.

Future Research Directions

• Structural Studies: Resolve 3D structures to understand ATPase-substrate interactions.

• CRISPR-Based Functional Analysis: Confirm AT3G28510’s role in stress adaptation using gene-edited plants.