At3g28580 Antibody

Key Features:

• Structural Role: AAA-ATPases typically form hexameric rings that hydrolyze ATP to drive conformational changes in substrate proteins .

• Expression Profile: Upregulated during oxidative stress, particularly under singlet oxygen ($^1O_2$) conditions .

Research Applications of the At3g28580 Antibody

This antibody has been employed in:

• Gene Expression Studies: Quantifying AT3G28580 protein levels in mutants (e.g., eds1) and transgenic lines (e.g., 35S::FLAG-RGGA) .

• Stress Response Analysis: Investigating its role in reactive oxygen species (ROS) signaling and pathogen defense .

• Interaction Studies: Identifying regulatory relationships with RNA-binding proteins like AtRGGA .

Role in Oxidative Stress

AT3G28580 is a marker for singlet oxygen ($^1O_2$) stress in Arabidopsis:

• Expression Data:

  Genotype	Fold Change (AT3G28580)	Significance
  Wildtype	Baseline	Control
  eds1 mutant	−2.42	Reduced expression
  35S::FLAG-RGGA	+2.50	Overexpression

This suggests AT3G28580 is modulated by the EDS1 pathway and redox-sensitive transcription factors .

Interaction with AtRGGA

The RNA-binding protein AtRGGA regulates AT3G28580 expression under drought and salt stress:

• Mechanism: AtRGGA binds poly(A−) RNA to stabilize transcripts like AT3G28580, enhancing AAA-ATPase activity during stress adaptation .

• Validation: Immunoblotting with the At3g28580 antibody confirmed protein accumulation in AtRGGA-overexpressing lines .

Data Table: AT3G28580 Expression Across Genotypes

Implications for Plant Biology

• Stress Adaptation: AT3G28580 contributes to ROS scavenging and organelle integrity under abiotic/biotic stress .

• Biotechnological Potential: Engineering AT3G28580 expression could improve crop resilience to environmental stressors.