At4g25835 Antibody

Shipped with Ice Packs
In Stock

Description

Development and Validation

Production Details:

  • Host Species: Mouse

  • Clonal Type: Monoclonal (combination panels for enhanced specificity)

  • Validation Metrics:

    • ELISA titer: 10,000 (equating to ~1 ng sensitivity in WB)

    • Epitope coverage: Designed for N-terminal, C-terminal, and non-terminal regions .

Quality Assurance:

  • AbClass™ Classification: "Hard" (indicating high stability and specificity) .

  • Cross-Reactivity: Specific to Arabidopsis thaliana; no reported cross-reactivity with other plant species .

Research Applications

Primary Uses:

  • Western Blot (WB): Detects endogenous expression levels of the AAA-type ATPase in Arabidopsis tissues.

  • Immunofluorescence (IF): Localizes the protein to subcellular compartments.

  • Immunohistochemistry (IHC): Tracks tissue-specific expression patterns.

Functional Studies:

  • Investigates roles in plant cellular processes such as vesicle trafficking, organelle biogenesis, and stress responses .

Limitations and Considerations

  • Variant Specificity: Designed for the canonical isoform (Q8RY66); may not detect splice variants.

  • Research Gaps: Limited peer-reviewed studies directly using this antibody highlight the need for further validation in mutant plant lines .

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At4g25835 antibody; F14M19.110AAA-ATPase At4g25835 antibody; EC 3.6.1.3 antibody
Target Names
At4g25835
Uniprot No.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2025 TheBiotek. All Rights Reserved.