At5g57480 Antibody

Overview of At5g57480 Antibody

The At5g57480 Antibody is a polyclonal antibody generated against a synthesized peptide derived from the At5g57480 protein. It is designed for applications such as Western blot (WB) and enzyme-linked immunosorbent assay (ELISA) . Key characteristics include:

• Host Species: Rabbit

• Clonality: Polyclonal

• Target Species: Arabidopsis thaliana

• Immunogen: Synthetic peptide corresponding to a region of the At5g57480 protein .

Biological Context of At5g57480

• Cellular transport processes (e.g., ABC transporters) .

• Stress response mechanisms, common in Arabidopsis membrane-associated proteins .
  Antibodies like At5g57480 are critical for mapping protein expression patterns under varying experimental conditions, such as abiotic stress or developmental stages.

Western Blotting

• Used to detect At5g57480 protein in Arabidopsis lysates, with a recommended dilution range of 1:500–1:2000 .

• Validates protein size (~30 kDa) and post-translational modifications.

ELISA

• Quantifies At5g57480 expression levels in plant tissues at a 1:20,000 dilution .

Localization Studies

• Preliminary data suggest cytoplasmic and membrane-associated localization, though further validation is required .

Comparative Analysis with Related Antibodies

This antibody’s specificity for At5g57480 distinguishes it from broader-spectrum plant antibodies .

Limitations and Future Directions

• Current Limitations: Limited peer-reviewed studies directly utilizing this antibody; most data derive from supplier documentation .

• Research Gaps: Functional characterization of the At5g57480 protein and its role in plant physiology.

• Recommendations: Pairing this antibody with CRISPR/Cas9 knockout models or transcriptomic analyses to elucidate its biological pathways.