ABCB10 Antibody

Key Features:

• Target: ABCB10 (UniProt ID: Q9NRK6) .

• Reactivities: Human, mouse, rat .

• Applications: Western blot (WB), immunoprecipitation (IP), immunohistochemistry (IHC) .

• Observed Molecular Weight: ~60 kDa (mature isoform) .

Key Applications in Research

ABCB10 antibodies have been instrumental in elucidating the transporter’s roles in mitochondrial biology:

Mitochondrial Iron Transport and Erythropoiesis

• ABCB10 physically interacts with mitoferrin-1 (Mfrn1/Slc25a37) to stabilize the iron transporter and enhance mitochondrial iron assimilation during erythroid differentiation .

• Immunoprecipitation studies in MEL cells confirmed endogenous ABCB10-Mfrn1 interaction only in differentiated erythroid cells .

Oxidative Stress Regulation

• ABCB10 is essential for mitigating mitochondrial reactive oxygen species (ROS). Its deletion in CD4+ T cells impairs cytokine production (e.g., TNFα, IL-2) and memory formation due to disrupted mitochondrial metabolism .

Lipid Binding and ATPase Activity

• ABCB10 binds cardiolipin with high affinity, regulating its ATPase activity. Phosphatidic acid also modulates activity, albeit less effectively .

Validation and Specificity

ABCB10 antibodies are validated across diverse platforms:

• Western Blot Validation: Detects endogenous ABCB10 in Jurkat and K-562 cells .

• Immunohistochemistry: Stains formalin-fixed human liver tissue .

Functional Defects in Knockout Models

• CD4+ T Cells: ABCB10 loss reduces TNFα/IL-2 production and impairs memory formation due to defective mitochondrial oxidative metabolism .

• Erythroid Cells: ABCB10 knockout mice die by embryonic day 12.5, underscoring its role in hemoglobin synthesis .

Interaction Networks

• ABCB10 does not interact with paralogs like Mfrn2 or ABCB6, highlighting specificity for Mfrn1 in erythroid cells .