ABCA11 Antibody

Introduction to ABCA1 Antibody

ABCA1 (ATP-binding cassette subfamily A member 1) is a transmembrane protein critical for cholesterol efflux and high-density lipoprotein (HDL) formation. ABCA1 Antibodies are tools used to study its role in lipid metabolism, atherosclerosis, and inflammation. These antibodies enable detection of ABCA1 in diverse experimental systems, including Western blot (WB), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FCM).

ABCA1 Antibody Characteristics

The following table summarizes key ABCA1 antibodies from commercial and academic sources:

Key Features:

• Sensitivity: Detects endogenous ABCA1 in human and murine samples (e.g., liver, macrophages, neuroblastoma cells).

• Specificity: Cross-reactivity with ABCA3/ABCA4/ABCA7 is excluded in select antibodies (e.g., Abcam’s [EPR27494-57]) .

• Validation: Knockout (KO) cell lines and tissues confirm specificity (e.g., Abcam’s AB.H10) .

Mechanisms and Functional Roles of ABCA1

ABCA1’s biological roles are critical for cardiovascular and metabolic health:

Cholesterol Efflux and HDL Formation

ABCA1 facilitates the transfer of cholesterol and phospholipids to apolipoprotein A-I (apoA-I), forming nascent HDL particles . Defects in ABCA1 cause Tangier disease, characterized by low HDL and atherosclerosis .

Anti-Inflammatory Activity

ABCA1 modulates lipid rafts in macrophages, suppressing Toll-like receptor 4 (TLR4) signaling and inflammatory cytokine production . Mice lacking ABCA1 show heightened responses to lipopolysaccharides (LPS) .

Atherosclerosis and Disease Models

In LDL receptor-deficient (LDLr⁻/⁻) mice with leukocyte-specific ABCA1 deletion, atherosclerotic lesions are larger, and macrophage infiltration increases . Plasma apoA-I levels are reduced, but HDL cholesterol remains unaffected .

Western Blotting (WB)

• HepG2 Cells: ABCA1 is detected at ~250–300 kDa, with induction by cholesterol or agonists like T0901317 .

• Mouse Liver: A 254 kDa band is observed in wild-type but absent in ABCA1 knockout (KO) mice .

Immunohistochemistry (IHC)

• Pancreas: Exocrine glandular cells show strong ABCA1 staining, while islet cells exhibit weak positivity .

• Lung Tissue: Antigen retrieval with TE buffer (pH 9.0) enhances detection .

Flow Cytometry (FCM)

• HepG2 Cells: Intracellular staining confirms ABCA1 localization after fixation/permeabilization .

Clinical and Therapeutic Implications

• Atherosclerosis: ABCA1-targeted therapies may reduce plaque formation by enhancing cholesterol efflux .

• Neurological Disorders: ABCA1’s role in lipid metabolism is linked to Alzheimer’s disease and age-related macular degeneration .