Customize ABCC11 Antibody

Description

What is the ABCC11 Antibody?

ABCC11 antibodies are immunodetection reagents targeting the ABCC11 protein, a multidrug resistance-associated transporter (MRP8) encoded by the ABCC11 gene. This protein facilitates ATP-dependent transport of lipophilic anions, including bile acids, steroid conjugates, and cyclic nucleotides (cAMP/cGMP), and is linked to earwax type determination and axillary odor formation .

Epitope Design and Host Systems

Epitope: Most ABCC11 antibodies target specific regions, such as the intracellular domain (residues A747–H795) or the N-terminal region (residues 343–372) .
Host: Polyclonal antibodies are typically raised in rabbits using KLH-conjugated synthetic peptides .
Validation: Specificity is confirmed via immunoblotting in ABCC11-transfected cells or tissues. For example, the antibody 09YT demonstrated specificity in transgenic mice and human apocrine glands .

Applications in Research

ABCC11 antibodies are widely used in:

Application	Details
Western Blot (WB)	Detects mature glycosylated ABCC11 (~154 kDa) in liver tissues and cancer cell lines .
Immunohistochemistry (IHC)	Localizes ABCC11 in human apocrine glands and tumor tissues, aiding studies on metastasis .
Drug Resistance Studies	Identifies ABCC11 overexpression in eribulin- or 5-fluorouracil-resistant breast/colon cancer cells .
Functional Studies	Links ABCC11 SNPs (e.g., 538G>A) to protein degradation and loss of function .

ABCC11 in Disease and Physiology

Drug Resistance: ABCC11 overexpression correlates with resistance to eribulin (breast cancer) and 5-fluorouracil (colon cancer) .
Metastasis: ABCC11-negative tumors exhibit higher lymph node metastasis and venous invasion rates .
Genetic Polymorphism: The ABCC11 538G>A SNP reduces protein stability via endoplasmic reticulum-associated degradation (ERAD), impacting axillary odor and earwax type .

Technical Considerations

Glycosylation Sensitivity: ABCC11’s mature glycosylated form is detectable via N-glycosidase treatment .
Species Reactivity: Most antibodies are validated for human samples, with predicted reactivity in bovine and canine models .
Storage: Long-term storage at -20°C in aliquots to prevent freeze-thaw cycles .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

ABCC11 antibody; MRP11 antibody; MRP12 antibody; At1g30420 antibody; F26G16.1 antibody; T4K22.1ABC transporter C family member 11 antibody; ABC transporter ABCC.11 antibody; AtABCC11 antibody; EC 7.6.2.2 antibody; ATP-energized glutathione S-conjugate pump 12 antibody; Glutathione S-conjugate-transporting ATPase 12 antibody; Multidrug resistance-associated protein 12 antibody

Target Names

ABCC11

Uniprot No.

Q9C8H1

Target Background

Function

Pump for glutathione S-conjugates.

Database Links

KEGG: ath:AT1G30420

STRING: 3702.AT1G30420.1

UniGene: At.51838

Protein Families

ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily

Subcellular Location

Membrane; Multi-pass membrane protein.

Tissue Specificity

Ubiquitous.

Q&A

Here’s a structured collection of FAQs tailored for academic researchers working with ABCC11 antibodies, synthesized from peer-reviewed studies and technical documentation:

Advanced Research Questions

How to resolve contradictory data on ABCC11 expression levels across studies?

Troubleshooting framework:

Variable	Impact on Results	Mitigation Strategy
Tissue heterogeneity	Apocrine vs. eccrine gland contamination	Laser-capture microdissection of glands
SNP 538G>A genotype	Reduced protein stability in 538A variants	Genotype samples before analysis
Post-translational modification	Glycosylation affects antibody binding	PNGase F treatment + deglycosylation controls

What experimental designs are optimal for studying ABCC11’s role in nucleotide analog resistance?

Stepwise approach:
- Model systems: Use ABCC11-transfected HEK293/MDCKII cells paired with vector controls .
- Functional assays:
  - Measure intracellular retention of 5-FU or PMEA via LC-MS .
  - Compare IC50 values between ABCC11-WT and knockout lines .
- Validation: Co-stain with apocrine markers (e.g., ApoD) to confirm tissue specificity .

How to optimize ABCC11 detection in low-abundance clinical samples?

Protocol refinement:
- Pre-amplification: Perform tyramide signal amplification (TSA) for IHC .
- Multiplexing: Combine ABCC11 antibody with fluorescent-conjugated secondary antibodies and nuclear counterstains (e.g., DAPI) .
- Quantitative analysis: Use densitometry software (e.g., ImageJ) with α-Tubulin/GAPDH normalization .

Technical Challenges & Solutions

Why does ABCC11 antibody performance vary between fresh vs. archived tissues?

Critical factors:
- Fixation time: Prolonged formalin fixation masks epitopes. Limit to 24–48 hours .
- Antigen retrieval: Optimize using high-pH Tris-EDTA buffer (pH 9.0) for archived FFPE blocks .
- Batch validation: Re-test antibody lot numbers on control tissues when switching suppliers .

How to distinguish ABCC11 from homologous transporters (e.g., ABCC12)?

Discrimination strategies:
- Epitope mapping: Use antibodies targeting non-conserved regions (e.g., ABCC11’s cytoplasmic loop vs. ABCC12’s N-terminus) .
- Knockdown controls: Perform siRNA-mediated ABCC11/ABCC12 co-silencing in validation experiments .

Data Interpretation Guidelines

How to contextualize conflicting reports on ABCC11’s role in cancer prognosis?

Case study: Colon cancer vs. breast cancer findings :

Cancer Type	ABCC11 Expression	Clinical Correlation	Proposed Mechanism
Colon cancer	Low expression	Shorter DFS with 5-FU therapy	Reduced drug efflux → cytotoxicity
Breast cancer	High expression	Resistance to nucleoside analogs	Enhanced efflux → chemoresistance

What orthogonal methods confirm ABCC11 antibody specificity?

Multi-modal validation:
- Proteomics: Immunoprecipitation followed by mass spectrometry .
- Genetic knockout: Compare signals in WT vs. ABCC11−/− cell lines .
- Functional blocking: Use ABCC11 inhibitors (e.g., MK571) to assess transport activity .

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ABCC11 Antibody