ABCI12 Antibody

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Description

Terminology Analysis

The term "ABCI12" does not correspond to:

  • Standard antibody nomenclature (e.g., WHO’s INN system for monoclonal antibodies)

  • Gene symbols (e.g., ABCA1, ABCC12, or ABCG2 in the ATP-binding cassette transporter family)

  • Clinical trial identifiers (e.g., ABC-12 refers to a biliary cancer study )

Similar Named Entities

NameRelevance to QuerySource Reference
ABCC12ATP-binding cassette transporter targeted by monoclonal antibody ab91453
ABC-12Clinical trial (NCT ISRCTN11210442) studying durvalumab + chemotherapy
CLEC12AC-type lectin domain protein studied as a COVID-19 biomarker
SC27Broad-spectrum SARS-CoV-2 antibody

Hypothetical Interpretations

  • Typographical error for ABCC12 (a multidrug resistance-associated protein).

  • Misinterpretation of ABC-12, a clinical trial identifier.

Research Recommendations

To resolve ambiguity, consider:

  1. Verify nomenclature with the Human Genome Organisation (HUGO) Gene Nomenclature Committee.

  2. Consult patent databases (e.g., USPTO, WIPO) for unpublished antibody designs.

  3. Review antibody engineering platforms (e.g., yeast/bacterial display libraries in ).

Standard Antibody Characterization

If "ABCI12" refers to a novel antibody, its characterization should include:

Key Parameters

ParameterExample from Analogous Antibodies
Target antigenSARS-CoV-2 spike protein (SC27 )
Neutralization potencyPRNT50 = 4.05 ng/mL (CSW1-1805 )
Structural epitopeRBD ridge binding (CSW1-1805 )
Clinical applicationsMalaria prophylaxis (ADCI-associated IgG )

Comparative Efficacy

AntibodyTargetIC50/EC50Clinical Phase
DurvalumabPD-L1N/AApproved
Humanized CXCL12 AbCXCL12Reduced CD8+ T-cell activation Preclinical

Product Specs

Buffer
Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M Phosphate-Buffered Saline (PBS), pH 7.4
Form
Liquid
Lead Time
14-16 week lead time (made-to-order)
Synonyms
ABCI12; At3g21580; MIL23.15; Protein ABCI12, chloroplastic; ABC transporter I family member 12; ABC transporter ABCI.12; AtABCI12
Target Names
ABCI12
Uniprot No.

Target Background

Database Links

KEGG: ath:AT3G21580

STRING: 3702.AT3G21580.1

UniGene: At.5658

Subcellular Location
Plastid, chloroplast. Membrane; Multi-pass membrane protein.

Q&A

When developing FAQs for the ABCI12 antibody in academic research, key considerations include validation protocols, experimental design robustness, and addressing reproducibility challenges. Below is a structured guide based on current best practices in antibody research and characterization.

Advanced Research Questions

How to resolve contradictory results between ABCI12 antibody batches?

  • Implement a three-tier validation framework:

    • Biochemical verification: Compare lot-specific affinity measurements via surface plasmon resonance

    • Functional testing: Replicate key experiments from previous publications

    • Epitope mapping: Identify binding regions through peptide array analysis

What strategies validate antibody performance across species?

  • Use phylogenetic alignment tools to assess epitope conservation

  • Test cross-reactivity in:

    • Primary cells from multiple species

    • Recombinant protein panels with ortholog sequences

  • Validate through independent application correlation (e.g., compare IHC results with mRNA FISH data)

How to design multi-omics studies using ABCI12 antibody?

Integration ApproachValidation MetricQuality Threshold
Proteomics-TranscriptomicsCorrelation coefficient (R²)≥0.7 for matched samples
IHC-spatial transcriptomicsRegional colocalization index≥80% spatial overlap
Flow cytometry-scRNAseqCluster-specific expression<5% false positive rate

Methodological Best Practices

7. Standardized characterization workflow for new ABCI12 applications:

  • Pre-validation:

    • Curate existing literature using antibody databases (CiteAb )

    • Analyze ABCI12 protein structure (post-translational modifications, isoforms)

  • Experimental validation:

    • Perform application-specific stress tests (e.g., prolonged fixation for IHC)

    • Establish quantitative performance metrics (signal-to-noise ratio ≥3:1)

  • Documentation:

    • Record batch-specific validation data using MIAPE-Ab guidelines

8. Addressing target polymorphism effects on antibody binding:

  • Maintain variant-specific cell line panels covering:

    • Common single amino acid polymorphisms

    • Alternative splicing isoforms

  • Implement dynamic binding assays to assess:

    • Conformational epitope stability (HDX-MS)

    • pH-dependent affinity changes (mimicking intracellular environments)

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