ABCB25 Antibody
Description
ABCB5 Overview
ABCB5 is an ATP-binding cassette transporter implicated in cancer stem cell maintenance, drug resistance, and immunomodulation . Two primary isoforms exist:
-
ABCB5FL (full transporter): Expressed in testes/prostate and linked to multidrug resistance.
-
ABCB5β (half-transporter): Ubiquitously expressed and associated with tumor-initiating cells in melanoma, breast cancer, and other malignancies .
Validated ABCB5 Antibodies
Commercial antibodies target specific ABCB5 epitopes, with applications in Western blot (WB), immunohistochemistry (IHC), and flow cytometry. Key examples include:
A02979 (Boster Bio)
-
Dilutions:
-
WB: 1:20,000
-
IHC: 1:200
-
-
Validation: Detects ABCB5 in paraffin-embedded tissues (Figure 1) .
PA5-114801 (Thermo Fisher)
-
Target: Endogenous ABCB5 across human, mouse, and rat samples .
-
Applications: WB, IHC, immunofluorescence.
ABIN238622 (Antibodies-Online)
Role in Cancer
-
Melanoma: ABCB5+ cells exhibit chemoresistance via IL-1β/IL-8 signaling . Knockdown (KD) of ABCB5 in A375/G3361 melanoma models reduced tumor growth by >3-fold in vivo .
-
Immunomodulation: ABCB5+ dermal mesenchymal cells prolong cardiac allograft survival via PD-1/IL-1RA pathways .
Therapeutic Targeting
-
Antibody 3C2-1D12: Blocks ABCB5-mediated drug efflux, sensitizing melanoma cells to chemotherapy .
-
Peptide Vaccines: ABCB5 extracellular loop 3-derived peptides (e.g., RFGAYLIQAGRMTPEG) induce CD8+ T-cell responses in preclinical models .
Technical Considerations
Limitations and Controversies
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Depletion of the mitochondrial, GSH-dependent persulfide oxygenase ETHE1 exacerbates the growth phenotype of atm3-1, suggesting that ATM3's physiological substrate contains both persulfide and glutathione. (PMID: 25006243)
- AtATM3 contributes to heavy metal resistance, potentially mediating the transport of glutamine synthetase-conjugated Cd(II) across the mitochondrial membrane in Arabidopsis. (PMID: 16461380)
- STABILIZED1 (STA1), a pre-mRNA splicing factor, is required for both splicing and unstable transcript turnover, and plays a significant role in plant responses to abiotic stress. (PMID: 16751345)
- Arabidopsis ATM3 may transport either (1) at least two distinct compounds or (2) a single compound required for both cytosolic iron-sulfur and molybdenum cofactor assembly machineries, but not iron itself. (PMID: 19710232)
