ABCG26 Antibody
Description
ABCG26 Protein Overview
ABCG26 (AT3G13220) is an Arabidopsis thaliana gene encoding a plasma membrane-localized ABC transporter essential for pollen exine formation and male fertility . Key features include:
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Function: Transports sporopollenin precursors from tapetal cells to developing pollen grains .
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Structure: Contains an N-terminal ATP-binding domain and a C-terminal transmembrane domain, typical of ABCG subfamily transporters .
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Phenotype: Knockout mutants (e.g., abcg26-1) exhibit pollen wall defects and sterility .
Research Tools for ABCG26 Analysis
While the provided sources do not explicitly describe a commercial ABCG26 antibody, studies employ alternative methods for protein characterization:
Genetic and Molecular Approaches
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T-DNA insertion mutants: Used to study ABCG26 loss-of-function phenotypes (e.g., abcg26-1, abcg26-2) .
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Complementation assays: Restored fertility in mutants via transgenic expression of ABCG26.1 or ABCG26.2 cDNA .
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Subcellular localization: Achieved using YFP-ABCG26 fusion constructs transiently expressed in protoplasts .
Antibody-Based Detection in Related Systems
For ABCG homologs like human ABCG2 (BCRP), antibodies such as ab130244 (Anti-BCRP/ABCG2 [1H2]) are widely used. Key applications include:
| Application | Details |
|---|---|
| Western Blot | Detects ~72 kDa band in HepG2, COS7, and NIH/3T3 cell lysates . |
| Immunofluorescence | Localizes ABCG2 in HeLa cells . |
| ELISA | Validates binding to recombinant ABCG2 fragments . |
Functional Insights
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Role in sporopollenin transport: ABCG26 mediates the export of lipidic sporopollenin precursors from tapetal cells to the anther locule .
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Localization: Plasma membrane-localized in tapetum cells during pollen development .
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Mutant phenotype: abcg26-1 mutants show collapsed pollen grains with defective exine layers .
Comparative Analysis of ABCG26 and ABCG2
| Feature | ABCG26 (Arabidopsis) | ABCG2 (Human) |
|---|---|---|
| Function | Pollen exine formation | Drug resistance, xenobiotic transport |
| Localization | Tapetum plasma membrane | Brain endothelium, gastrointestinal tract |
| Antibody Tools | No commercial antibody described | ab130244 (validated for WB, IF, ELISA) |
| Structure | Predicted homodimer | Cryo-EM structure resolved (PDB: 5NIV) |
Future Directions
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Antibody development: Generation of ABCG26-specific antibodies would enable direct protein quantification and tissue localization studies.
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Biochemical profiling: Identify accumulated sporopollenin precursors in abcg26 mutants to elucidate transported substrates .
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Structural studies: Apply cryo-EM techniques, as used for ABCG2 , to resolve ABCG26’s molecular architecture.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies have shown that ABCG26-exported polyketides are transported from tapetal cells, where they contribute to the formation of the sporopollenin backbone. This process occurs in coordination with the trafficking of other constituents, prior to the programmed cell death of the tapetum. PMID: 25415974
- Research has demonstrated that AtABCG26, the Arabidopsis homolog of ABCG26, is essential for pollen development. Mutations in AtABCG26 result in significantly reduced pollen grain production, leading to male sterility. PMID: 21696844
- Arabidopsis thaliana ABCG26 plays a crucial role in transferring sporopollenin lipid precursors from tapetal cells to the anther locules, directly supporting exine formation on the pollen surface. PMID: 21223384
- Research indicates that the ABC transporter, WBC27 (AT3G13220), plays a significant role in the formation of the Arabidopsis exine. This transporter likely facilitates the translocation of lipidic precursors of sporopollenin from the tapetum to developing microspores. PMID: 21205178
- ABCG26 is indispensable for normal male fertility, exine formation, and pollen maturation. PMID: 20732973
KEGG: ath:AT3G13220
STRING: 3702.AT3G13220.1
Q&A
Here’s a structured FAQ collection for researchers investigating ABCG2 antibodies in academic contexts, synthesized from peer-reviewed methodologies and experimental insights:
Advanced Research Questions
How to resolve contradictory ABCG2 localization data between studies?
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Troubleshooting framework:
What functional assays best characterize ABCG2 modulation in drug-resistant cancers?
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Integrated approach:
How to design CRISPR-Cas9 experiments to study ABCG2 regulation?
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Critical steps:
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Design sgRNAs targeting exon 2 (common to all splice variants).
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Include ABCG2-GFP reporter lines to monitor transcriptional dynamics.
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Validate off-target effects via RNA-seq in KO clones.
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Methodological Notes
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Antibody validation: Always pair antibody-based detection with functional readouts (e.g., drug IC50 shifts ≥5-fold post-Ko143 treatment ).
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Data interpretation: Account for ABCG2 polymorphisms (e.g., C421A) that alter protein stability and substrate affinity .
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Ethical reporting: Disclose antibody validation steps per Nature Publishing guidelines, including clone ID, dilution, and validation controls .
