ABCE2 Antibody

ABCE2 Overview

ABCE2 is a member of the ABC transporter superfamily, which utilizes ATP hydrolysis to drive substrate transport across cellular membranes. Unlike ABCG2 (a well-studied transporter linked to multidrug resistance), ABCE2 is primarily implicated in ribosomal biogenesis and translation regulation.

• Function:

  • In Arabidopsis thaliana, ABCE2 interacts with ribosomal proteins and translation initiation factors, supporting its conserved role in eukaryotic translation .

  • Human ABCE2 homologs are hypothesized to participate in ribosome recycling, though direct evidence remains limited .

• Structural Features:

  • ABCE2 contains twin ATP-binding domains and a FeS cluster critical for its enzymatic activity .

ABCE2 Antibody Development and Applications

Despite its biological significance, commercial or research-grade antibodies specifically targeting ABCE2 are not widely documented in current literature. Most available data focus on ABCG2, a related transporter with established roles in cancer drug resistance.

Key Challenges in ABCE2 Antibody Research

• Limited Commercial Availability: No validated ABCE2 antibodies are listed in major antibody databases (e.g., Cell Signaling Technology, R&D Systems) based on reviewed sources .

• Species Specificity: Studies on ABCE2 antibodies are confined to plant models (Arabidopsis), with no reported applications in human systems .

Comparative Analysis: ABCE2 vs. ABCG2 Antibodies

ABCE2 in Plant Studies

• Arabidopsis ABCE2:

  • Co-immunoprecipitation assays identified ABCE2 interactors, including ribosomal proteins and FeS cluster assembly factors .

  • Silencing ABCE2 disrupts translation, underscoring its essential role .

ABCG2 in Human Disease

• Cancer:

  • Overexpression in head and neck squamous cell carcinoma (HNSCC) correlates with drug resistance and poor prognosis .

  • Antibodies like #4477 (Cell Signaling Technology) enable detection of ABCG2 in Western blotting and immunohistochemistry .

• COVID-19:

  • ACE2-targeting antibodies (e.g., 3E8) block SARS-CoV-2 entry, showcasing the therapeutic potential of transporter-focused antibodies .

Future Directions

• ABCE2-Specific Antibody Development:

  • Epitope mapping and hybridoma technology (as used for ACE2 antibody 3E8 ) could be adapted to generate ABCE2 antibodies.

  • Structural studies (e.g., cryo-EM) may reveal conserved ABCE2 epitopes for antibody design .

• Therapeutic Potential:

  • ABCE2 modulation could address translation-related disorders, though current tools remain insufficient.