ABCG6 Antibody
Description
Definition and Target
ABCG6 (ATP-binding cassette subfamily G member 6) is a half-transporter protein belonging to the ABCG subgroup. In Leishmania infantum, ABCG6 (LiABCG6) localizes to the plasma membrane and plays a role in phospholipid trafficking and drug resistance . The ABCG6 antibody is a polyclonal or monoclonal reagent designed to bind specifically to this protein, enabling its detection in assays like Western blotting, immunofluorescence, and functional studies.
Functional Role of ABCG6
ABCG6 contributes to two primary biological processes:
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Drug Resistance: Overexpression of LiABCG6 reduces intracellular accumulation of antileishmanial drugs (e.g., miltefosine and sitamaquine), conferring resistance .
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Phospholipid Transport: LiABCG6 mediates the efflux of short-chain fluorescent phospholipid analogs (phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine), suggesting a role in lipid homeostasis .
Drug Resistance Mechanisms
| Parameter | Details |
|---|---|
| Drugs Affected | Miltefosine, sitamaquine |
| Resistance Mechanism | Reduced drug accumulation via efflux |
| Experimental Model | Leishmania infantum promastigotes overexpressing LiABCG6 |
Phospholipid Trafficking
| Lipid Analogue | Accumulation Reduction (%) | Method Used |
|---|---|---|
| Fluorescent PC | 65 ± 8 | Flow cytometry |
| Fluorescent PE | 72 ± 6 | Fluorescence microscopy |
| Fluorescent PS | 58 ± 5 | Spectrophotometry |
These findings indicate ABCG6’s dual role in xenobiotic resistance and endogenous lipid transport .
Antibody Applications
ABCG6 antibodies have been utilized in:
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Localization Studies: Confirming plasma membrane localization in Leishmania via immunofluorescence .
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Functional Assays: Validating protein expression in drug-resistant strains through Western blotting .
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Mechanistic Insights: Linking transporter activity to intracellular drug kinetics using fluorescence-based assays .
Comparative Context with Other ABC Transporters
While ABCG6 is specific to Leishmania, homologous transporters in humans (e.g., ABCB1, ABCG2) are well-documented in cancer multidrug resistance . Unlike human ABCB6, which is dispensable for heme biosynthesis , ABCG6’s primary function in pathogens underscores its evolutionary divergence.
Limitations and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
