CYP707A1 Antibody

Overview of CYP707A1 Antibody

The CYP707A1 antibody is a specialized immunological tool designed to detect and study the CYP707A1 protein, a member of the cytochrome P450 family in plants. This enzyme, encoded by the CYP707A1 gene, functions as an abscisic acid (ABA) 8′-hydroxylase, catalyzing the oxidative degradation of ABA into phaseic acid (PA) to regulate ABA homeostasis . The antibody is critical for investigating ABA-mediated processes such as seed dormancy, drought responses, and stress signaling .

Table 1: Epitope Characterization

Table 2: Antibody Performance in Research Studies

Role in ABA Homeostasis

CYP707A1 antibody has been pivotal in elucidating ABA catabolism pathways. For example:

• Seed Dormancy: cyp707a1 mutants exhibited hyperdormancy due to ABA overaccumulation (70× higher in triple mutants) .

• Drought Response: HDA9-ABI4 complex represses CYP707A1 transcription under drought, as shown via ChIP .

Osmotic Stress Signaling

VIP1, a bZIP transcription factor, directly binds CYP707A1 promoter fragments during rehydration, confirmed by EMSA and ChIP .

Pathogen Interactions

Under high humidity, CYP707A1 and CYP707A3 co-regulate ABA depletion to restrict bacterial water-soaking in Arabidopsis .

Technical Considerations

• Cross-Reactivity: Potential reactivity with homologs (e.g., CYP707A2/A3) requires validation via mutant lines .

• Sample Preparation: Microsomal fractions enriched for membrane-bound CYP707A1 yield optimal results .