CYP707A2 Antibody
Description
Antibody Overview
The CYP707A2 antibody targets abscisic acid 8′-hydroxylase 2, an enzyme encoded by the CYP707A2 gene (AT2G29090) in Arabidopsis thaliana. This cytochrome P450 enzyme catalyzes ABA hydroxylation, a key step in ABA catabolism .
Biological Role of CYP707A2
CYP707A2 regulates ABA levels during seed maturation and germination:
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Seed Dormancy: Mutants lacking CYP707A2 (e.g., cyp707a2-1) exhibit hyperdormancy due to delayed ABA degradation, with ABA levels 5–6× higher than wild-type seeds .
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Nitrate Signaling: Nitrate induces CYP707A2 expression, linking nutrient availability to ABA catabolism and dormancy release .
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Environmental Stress: Drought upregulates CYP707A2, while glucose represses it, modulating ABA dynamics during stress responses .
A. Mechanistic Insights
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Promoter Analysis: A 1.9-kb CYP707A2 promoter region (−1,661 to −1,373) is essential for nitrate-induced expression, validated via GUS reporter assays .
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Protein-DNA Interaction: Chromatin immunoprecipitation (ChIP) confirmed NLP8 transcription factor binding to the CYP707A2 promoter under nitrate treatment .
B. Mutant Phenotypes
| Genotype | ABA Level in Dry Seeds | Germination Defect |
|---|---|---|
| cyp707a2-1 | 5–6× WT | Delayed ABA reduction |
| cyp707a1 cyp707a2 | 50× WT | Severe hyperdormancy |
| cyp707a1a2a3 triple | 70× WT | Non-germinating without after-ripening |
C. Expression Dynamics
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Tissue Specificity: CYP707A2 mRNA localizes to embryo endosperm and vascular tissues during late seed maturation .
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Induction by ABA: Exogenous ABA upregulates CYP707A2 within 6 hours of seed imbibition, accelerating ABA degradation .
Technical Validation
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Western Blot: Used to correlate CYP707A2 transcript levels with protein abundance under glucose treatment .
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Localization Studies: In situ hybridization revealed dynamic CYP707A2 expression shifts between seed maturation and imbibition stages .
Implications for Agriculture
Modulating CYP707A2 activity could enhance crop resilience:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Genetic studies have demonstrated that the promotion of seed germination by nitrate, mediated by NLP8, requires the presence of CYP707A2. PMID: 27731416
- Research has shown that the delay in seed germination and seedling development induced by glucose is directly linked to the suppression of ABA catabolism through the repression of CYP707A2 gene expression. PMID: 21883251
- CYP707A2 transcript levels exhibit a gradual increase from late maturation to mature dry seed, suggesting a distinct role for this gene in seed development and post-germination growth. PMID: 16543410
- CYP707A2 has been identified as a central player in the nitrate-mediated control of ABA levels during seed development and germination in Arabidopsis thaliana. PMID: 19074630
