CYP707A3 Antibody

Functional Role of CYP707A3

CYP707A3 regulates ABA homeostasis by converting active ABA into phaseic acid, impacting plant responses to dehydration, rehydration, and pathogen interactions . Key findings include:

• Drought Response: CYP707A3 expression is rapidly induced during dehydration and rehydration, modulating ABA levels to fine-tune stomatal closure and drought tolerance .

• Pathogen Defense: Under high humidity, CYP707A3-mediated ABA depletion restricts bacterial water-soaking by promoting stomatal opening, a defense mechanism exploited by Pseudomonas syringae via effector proteins .

• Seed Development: CYP707A3, alongside CYP707A1, is epigenetically regulated by the H3K27me3 demethylase REF6 to control ABA levels during seed maturation .

Applications of CYP707A3 Antibody in Research

The antibody enables precise detection and quantification of CYP707A3 protein levels across experimental conditions. Key applications include:

Protein Expression Analysis

• Mutant Validation: Used to confirm CYP707A3 protein absence in cyp707a3 knockout mutants (e.g., cyp707a3-1), which exhibit elevated ABA levels and enhanced drought tolerance .

• Stress Response Studies: Quantifies CYP707A3 induction during dehydration/rehydration cycles and pathogen challenges .

Localization and Interaction Studies

• Tissue-Specific Expression: Immunohistochemistry reveals vascular tissue and guard cell localization, aligning with its role in systemic ABA signaling .

• Promoter Binding Assays: Chromatin immunoprecipitation (ChIP) identifies transcription factors (e.g., VIP1, CAMTA3) regulating CYP707A3 expression .

Key Research Findings Enabled by CYP707A3 Antibody

Validation and Specificity

• Specificity: Antibody validation includes Western blotting against cyp707a3 mutants (no signal) and overexpression lines (strong signal) .

• Cross-Reactivity: No cross-reactivity with other CYP707A isoforms (e.g., CYP707A1/A2/A4) confirmed via peptide-blocking assays .

Future Directions

• Structural Studies: Antibody-assisted crystallography to resolve CYP707A3’s active site for inhibitor design.

• Pathogen Resistance Engineering: Targeting CYP707A3 expression to enhance bacterial resistance in crops via CRISPR/Cas9 .