ABCB10 Antibody

Definition and Biological Context

ABCB10 antibody is a research tool targeting the ATP-binding cassette sub-family B member 10 (ABCB10), a mitochondrial inner membrane transporter critical for erythropoiesis, oxidative stress resistance, and mitochondrial iron metabolism . ABCB10 facilitates heme biosynthesis by stabilizing mitoferrin-1 (a mitochondrial iron importer) , exports biliverdin , and regulates cardiolipin-dependent ATPase activity . Antibodies against ABCB10 are used to detect its expression, localization, and functional interactions in cellular and disease models.

Key Applications and Validation

ABCB10 antibodies are validated for techniques including:

Validation Data:

• Specificity: Recognizes recombinant ABCB10 fragments (aa 450–C terminus) and native protein in mitochondrial lysates .

• Cross-reactivity: Confirmed in human, mouse, rat, and pig samples .

Disease Associations

• Cardiac Dysfunction: Cardiomyocyte-specific ABCB10 knockout mice develop fibrosis, mitochondrial ROS overload, and ferroptosis due to lysosomal iron accumulation .

• Erythropoiesis: ABCB10 deficiency disrupts hemoglobin synthesis, linking it to anemia pathologies .

Technical Considerations

• Epitope: Most antibodies target C-terminal regions (e.g., aa 450–738) .

• Buffer Stability: Store at -20°C in PBS with 0.02% sodium azide .

• Dilution Range:

  • WB: 1:500–1:10,000 .

  • IP: 0.5–4.0 µg per mg lysate .

Functional Implications in Disease Models

• Ferroptosis: ABCB10 knockdown increases lysosomal Fe²⁺ and lipid peroxidation, reversed by iron chelators .

• Oxidative Stress: ABCB10-null cells show elevated mitochondrial ROS and 4-HNE adducts, confirming its antioxidant role .

• Therapeutic Potential: Targeting ABCB10 could mitigate ischemia-reperfusion injury or anemia .

Limitations and Future Directions

• Unresolved Substrate: The physiological substrate(s) of ABCB10 remain unidentified despite structural advances .

• Tissue-Specific Roles: Non-erythroid functions (e.g., cardiac protection) require deeper mechanistic exploration .