Acaca Antibody
Description
Definition and Target
Acaca Antibody is a monoclonal or polyclonal antibody designed to specifically bind to ACACA, the enzyme encoded by the ACACA gene. ACACA catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid biosynthesis .
Applications in Research
Acaca Antibodies are widely used for:
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Western Blot (WB): Detects ACACA in lysates from human and mouse cell lines (e.g., A431, HCT-116, HepG2, C2C12) .
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Immunohistochemistry (IHC) and Immunocytochemistry (ICC): Localizes ACACA in tissue sections and cultured cells .
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Enzyme-Linked Immunosorbent Assay (ELISA): Quantifies ACACA levels in biological samples .
Example Data from Western Blot
| Cell Line | Species | Band Size (kDa) | Citation |
|---|---|---|---|
| A431 (human epithelial carcinoma) | Human | 250 | |
| C2C12 (mouse myoblast) | Mouse | 250 |
Role in Metabolic Regulation
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ACACA inhibition reduces lipid accumulation by activating the AMPK-PPARα-CPT1A pathway, improving mitochondrial function in non-alcoholic fatty liver disease (NAFLD) models .
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In pancreatic alpha-cells, ACACA knockout (gluACC1KO) impairs glucagon secretion during hypoglycemia, highlighting its role in metabolic homeostasis .
Oncology Applications
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ACACA is overexpressed in cancers such as colon carcinoma, where it is a potential therapeutic target. Citrus lemon-derived nanovesicles targeting ACACA show antitumor effects .
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Studies using Acaca Antibodies revealed altered lipid metabolism in hepatocellular carcinoma cells exposed to diethylnitrosamine .
Key Citations
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Lipids in Health and Disease (2021): ACACA’s involvement in hepatocellular carcinoma lipidosis .
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Molecular Medicine Reports (2023): Proteomic studies of ACACA in bacterial biofilms .
Future Directions
Research is exploring ACACA inhibitors (e.g., targeting BC/CT domains) for metabolic disorders and cancer . Antibody-based diagnostics may enable personalized therapies by correlating ACACA levels with disease progression .
Product Specs
Target Background
- Inhibition of ACC1 activity impairs glucose-stimulated insulin secretion by inhibiting glucose metabolism rather than lipid metabolism. PMID: 18381287
- Research highlights the site-specific impacts of hypothalamic acetyl-CoA carboxylase activation in leptin's anorectic signaling cascade. PMID: 17956983
- Consistent with pathways of hepatic triglyceride synthesis, expression of diacylglycerol acyltransferase-1 and -2 was increased, as were delta-5- and delta-6- fatty acid desaturase mRNA levels. PMID: 18280001
- The citrate produced by mitochondria may modulate ACC phosphorylation in the hypothalamus, controlling food intake and coordinating a multiorgan network that controls glucose homeostasis and energy uptake through the adrenergic system. PMID: 18534630
- Data supports a potential role for Acetyl-CoA carboxylases (ACC) 1 and 2 in the development of obesity and diabetes in rats. These findings indicate major differences in ACC tissue distribution between humans and rats. PMID: 19618481
