ACBP2 Antibody
Description
Definition and Target
ACBP2 Antibody specifically binds to ACBP2, a member of the acyl-CoA-binding protein family that facilitates intracellular transport of acyl-CoA esters and regulates lipid metabolism. ACBP2 is distinct from other isoforms (e.g., ACBP1, ACBP/DBI) due to its unique expression patterns and functional roles .
Plant Studies
In Arabidopsis thaliana, ACBP2-specific antibodies revealed its expression in developing embryos, where it collaborates with ACBP1 to mediate lipid transfer during early embryogenesis. Key findings include:
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Embryo Lethality: Double mutants (acbp1acbp2) failed to develop viable embryos, underscoring ACBP2's necessity in lipid mobilization for seed development .
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Lipid Profiling: Siliques of acbp2 mutants showed reduced polyunsaturated phospholipids (e.g., phosphatidylcholine) and accumulation of galactolipids, indicating disrupted lipid homeostasis .
Parasite Studies
In Toxoplasma gondii, ACBP2 antibodies were used to validate mitochondrial localization and roles in cardiolipin metabolism:
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Mitochondrial Dysfunction: ACBP2-deficient parasites exhibited impaired growth, disrupted cardiolipin synthesis, and apoptosis-like death under stress .
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Virulence Attenuation: ACBP2 knockout reduced virulence in mice, linking lipid metabolism to pathogenicity .
Applications in Research
ACBP2 Antibody has been employed in diverse experimental techniques:
Key Research Findings
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Essential Role in Embryogenesis: ACBP2 is indispensable for lipid transfer during early seed development in plants .
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Mitochondrial Lipid Metabolism: In Toxoplasma, ACBP2 maintains cardiolipin levels critical for mitochondrial integrity and virulence .
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Therapeutic Potential: Targeting ACBP2 in pathogens like Toxoplasma could inform antiparasitic strategies .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- ACBP2, exhibiting 76.9% amino acid homology to ACBP1, does not appear to be involved in the freezing response. PMID: 20404541
- Research suggests that ACBP2 plays a positive role in promoting abscissic acid signaling during germination, seedling development, and the drought response. PMID: 22788984
- ACBP2 mediates tolerance to Cd(II)-induced oxidative stress by interacting with two protein partners, AtFP6 and lysoPL2. PMID: 20657176
- ACBP2 binds lysoPL2, promoting the degradation of lysophosphatidylcholine in response to Cd-induced oxidative stress. PMID: 20345607
- ACBP1 and ACBP2 are essential for lipid transfer during early embryogenesis in Arabidopsis. PMID: 20345632
- ACBP2 interacts with AtFP6. An ACBP2 derivative lacking ankyrin repeats failed to interact with AtFP6, indicating that these repeats mediate the interaction. Plants overexpressing ACBP2 or AtFP6 exhibited greater tolerance to Cd(II) than wild-type plants. PMID: 18823312
