At1g12010 Antibody

At1g12010 Gene and Protein Overview

The At1g12010 locus in Arabidopsis encodes a glycine-rich protein (GRP) characterized by repetitive glycine motifs, which are often associated with structural roles in cell walls or membranes. GRPs are known to participate in stress responses, including pathogen defense and abiotic stress tolerance .

Key features:

• Gene ID: AT1G12010 (Arabidopsis Genome Initiative).

• Protein class: Glycine-rich protein family.

• Function: Hypothesized to stabilize RNA or contribute to cellular integrity during stress .

Antibody Development and Validation

While no study directly describes the At1g12010 antibody’s generation, typical workflows for plant protein antibodies include:

• Immunogen design: A peptide sequence unique to the At1g12010 protein (e.g., residues 50–70) is synthesized.

• Host immunization: Rabbits or mice are immunized, followed by hybridoma generation for monoclonal antibodies .

• Validation: Western blotting, immunoprecipitation, and immunohistochemistry using knockout mutants as negative controls .

Example validation metrics:

3.1. Role in Clubroot Disease

In Plasmodiophora brassicae-infected Arabidopsis, At1g12010 expression is significantly upregulated during early infection stages (Table 1) .

Table 1: Differential Expression of At1g12010 in Infected vs. Control Roots

This suggests a role in early defense signaling, potentially via interaction with pathogen-derived molecules .

3.2. Stress Response Profiling

Proteomic studies under cold stress identified At1g12010 as part of a co-expression network involving:

• Cellular signaling: Interaction with calreticulin (AT5G07340) and MAP kinases .

• Redox regulation: Association with peroxidases (AT4G11310) .

Challenges and Future Directions

• Specificity: Glycine-rich domains may cross-react with unrelated GRPs .

• Therapeutic potential: Engineered plant antibodies (e.g., single-domain variants) could enhance crop resilience .