Acetyl-HIST1H1E (K16) Antibody
Description
Definition and Target
The Acetyl-HIST1H1E (K16) Antibody is a polyclonal rabbit-derived antibody designed to specifically recognize the acetylation of lysine 16 (K16) on the histone H1.4 protein (HIST1H1E). Histone H1.4 is a linker histone critical for chromatin fiber structure and gene regulation. Acetylation at K16 neutralizes the positive charge of lysine, promoting chromatin relaxation and transcriptional activation .
Research Applications
a. Chromatin Structure Studies
The antibody is validated for ChIP assays to map acetylation sites on chromatin. For example, it can detect acetylation at the beta-Globin promoter in sodium butyrate-treated HeLa cells, as demonstrated by CUSABIO .
b. Epigenetic Regulation
Acetylation of HIST1H1E (K16) is linked to transcriptional activation. Studies using this antibody have shown its utility in analyzing histone acetylation in cancer and developmental contexts .
Suppliers and Availability
| Supplier | Product Code | Conjugate | Price Range (USD) |
|---|---|---|---|
| Biomatik | CAC15239 | Unconjugated | $214–$319 |
| CUSABIO | CSB-PA010378PA16acHU | Unconjugated | $319 |
| Creative Biolabs | Recombinant Antibody | Inquire | Inquire |
Research Findings
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies have shown the presence of histones H1.2 and H1.4 in MDA-MB-231 metastatic breast cancer cells. Phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during the M phase, suggesting a cell cycle-dependent nature of these events. PMID: 26209608
- Research has identified and confirmed alterations in HIST1H1E protein within the postsynaptic density in schizophrenia. PMID: 25048004
- The N-terminal domain of H1 is a crucial determinant of the affinity and specificity of H1-chromatin interactions. PMID: 22425985
- PKA-mediated H1.4S35 phosphorylation detaches H1.4 from mitotic chromatin, suggesting its involvement in specific mitotic functions. PMID: 21852232
- Allele-specific underacetylation of histone H4 downstream from the promoter is associated with X-inactivation in human cells. PMID: 12498347
- The lysine residue adjacent to the phosphorylation site found on the serine residue on the H1.4 peptide KARKSAGAAKR has been observed to be methylated, raising the question of whether the hypothesized "methyl/phos" switch could be extended to linker histones. PMID: 15595731
- Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins PMID: 19144645
