Acetyl-HIST1H3A(K18) Recombinant Monoclonal Antibody
Description
Applications and Validation
The antibody is validated for diverse techniques, with optimized dilutions and specificities:
Specificity Data:
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Peptide Array Tests: Strong binding to K18ac peptide (ab24003); no cross-reactivity with acetyl K9, K14, K23, K27, or K36 peptides .
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Negative Controls: No signal when using unmodified H3 or unrelated acetylated peptides .
Research Significance and Mechanistic Insights
Acetylation of H3K18 is associated with:
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Gene Activation: Facilitates transcription by loosening chromatin structure .
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Epigenetic Regulation: Critical in development, differentiation, and stress responses .
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Therapeutic Targets: Sodium butyrate (HDAC inhibitor) increases H3K18ac levels, detected via this antibody .
Case Study:
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Sodium Butyrate Treatment: ChIP and WB show elevated H3K18ac in HeLa cells, confirming HDAC inhibition efficacy .
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Trichostatin A (TSA) Effects: Trichostatin A (another HDAC inhibitor) upregulates H3K18ac, validated by WB .
Comparative Analysis of Clones
Product Specs
The acetyl-HIST1H3A recombinant monoclonal antibody is produced using a robust expression system. The antibody-encoding gene is inserted into expression vectors, which are then delivered into host cells via polyethyleneimine-mediated transfection. The host cells are then cultured to facilitate the production and secretion of the antibodies. Following affinity chromatography purification, the antibody's functionality is rigorously assessed through ELISA, IHC, IF, and FC tests. These tests confirm the antibody's ability to specifically recognize the human acetylated HIST1H3A protein.
Acetylated HIST1H3A serves as a critical epigenetic marker linked to the activation of gene expression. It plays a crucial role in modulating chromatin structure, making genes more accessible for transcription. This process is essential for a wide range of cellular functions, including development, differentiation, and the response to environmental cues.
Target Background
Histone H3 is a core component of nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries, including those involved in transcription, DNA repair, replication, and maintenance of chromosomal stability. Histones play a critical role in regulating these processes.
The accessibility of DNA is carefully regulated through a complex set of post-translational modifications of histones, often referred to as the histone code. This code involves various modifications, including acetylation, methylation, phosphorylation, and ubiquitination, which influence the structure and function of nucleosomes, ultimately affecting gene expression.
- Research suggests a mechanism for epigenetic regulation in cancer involving the induction of E3 ubiquitin ligase NEDD4-dependent histone H3 ubiquitination. PMID: 28300060
- Elevated expression of H3K27me3 during a patient's clinical course can be helpful in determining whether tumors are heterochronous. PMID: 29482987
- A recent study identified JMJD5, a Jumonji C (JmjC) domain-containing protein, as a Cathepsin L-type protease that mediates histone H3 N-tail proteolytic cleavage under stress conditions leading to a DNA damage response. PMID: 28982940
- Evidence suggests that Ki-67 antigen proliferative index has significant limitations, and phosphohistone H3 (PHH3) presents itself as an alternative proliferative marker. PMID: 29040195
- This research highlights cytokine-induced histone 3 lysine 27 trimethylation as a mechanism that stabilizes gene silencing in macrophages. PMID: 27653678
- Data reveals that in the early developing human brain, HIST1H3B comprises the largest proportion of H3.1 transcripts among H3.1 isoforms. PMID: 27251074
- In a series of 47 diffuse midline gliomas, histone H3-K27M mutation was found to be mutually exclusive with IDH1-R132H mutation and EGFR amplification, rarely co-occurring with BRAF-V600E mutation, and was commonly associated with p53 overexpression, ATRX loss, and monosomy 10. PMID: 26517431
- Studies have shown that histone chaperone HIRA co-localizes with viral genomes, binds to incoming viral, and deposits histone H3.3 onto these. PMID: 28981850
- These experiments demonstrate that PHF13 binds specifically to DNA and to two types of histone H3 methyl tags (lysine 4-tri-methyl or lysine 4-di-methyl) where it functions as a transcriptional co-regulator. PMID: 27223324
- Hemi-methylated CpGs DNA recognition activates UHRF1 ubiquitylation towards multiple lysines on the H3 tail adjacent to the UHRF1 histone-binding site. PMID: 27595565
- This study describes, for the first time, the MR imaging features of pediatric diffuse midline gliomas with histone H3 K27M mutation. PMID: 28183840
- Approximately 30% of pediatric high grade gliomas (pedHGG) including GBM and DIPG harbor a lysine 27 mutation (K27M) in histone 3.3 (H3.3) which is correlated with poor outcome and was shown to influence EZH2 function. PMID: 27135271
- The presence of H3F3A K27M mutation in adult cerebellar HGG is not uncommon. PMID: 28547652
- Research suggests that lysyl oxidase-like 2 (LOXL2) is a histone modifier enzyme that removes trimethylated lysine 4 (K4) in histone H3 (H3K4me3) through an amino-oxidase reaction. PMID: 27735137
- Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication. PMID: 27341472
- The SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing. PMID: 27614073
- Data suggests that binding of helical tail of histone 3 (H3) with PHD ('plant homeodomain') fingers of BAZ2A or BAZ2B (bromodomain adjacent to zinc finger domain 2A or 2B) requires molecular recognition of secondary structure motifs within H3 tail and could represent an additional layer of regulation in epigenetic processes. PMID: 28341809
- The results indicate a novel mechanism by which Kdm4d regulates DNA replication by reducing the H3K9me3 level to facilitate formation of preinitiation complex. PMID: 27679476
- This research highlights histone H3 modifications caused by traffic-derived airborne particulate matter exposures in leukocytes. PMID: 27918982
- A key role of persistent histone H3 serine 10 or serine 28 phosphorylation in chemical carcinogenesis through regulating gene transcription of DNA damage response genes has been identified. PMID: 27996159
- hTERT promoter mutations are frequently observed in medulloblastoma and are associated with older patients, prone to recurrence, and located in the right cerebellar hemisphere. In contrast, histone 3 mutations do not appear to be present in medulloblastoma. PMID: 27694758
- AS1eRNA-driven DNA looping and activating histone modifications promote the expression of DHRS4-AS1 to economically control the DHRS4 gene cluster. PMID: 26864944
- Data suggests that nuclear antigen Sp100C is a multifaceted histone H3 methylation and phosphorylation sensor. PMID: 27129259
- The authors propose that histone H3 threonine 118 phosphorylation via Aurora-A alters the chromatin structure during specific phases of mitosis to promote timely condensin I and cohesin disassociation, which is essential for effective chromosome segregation. PMID: 26878753
- Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its H3 histone recognition. PMID: 27045799
- The functional importance of H3K9me3 in hypoxia, apoptosis, and repression of APAK has been demonstrated. PMID: 25961932
- In conclusion, the authors confirmed that histone H3 is a real substrate for GzmA in vivo in Raji cells treated with staurosporin. PMID: 26032366
- Circulating H3 levels have been found to correlate with mortality in sepsis patients and inversely correlate with antithrombin levels and platelet counts. PMID: 26232351
- Data shows that double mutations on the residues in the interface (L325A/D328A) decreases the histone H3 H3K4me2/3 demethylation activity of lysine (K)-specific demethylase 5B (KDM5B). PMID: 24952722
- Research indicates that minichromosome maintenance protein 2 (MCM2) binding is not required for incorporation of histone H3.1-H4 into chromatin but is important for stability of H3.1-H4. PMID: 26167883
- Data suggests that histone H3 lysine methylation (H3K4me3) plays a crucial mechanistic role in leukemia stem cell (LSC) maintenance. PMID: 26190263
- PIP5K1A modulates ribosomal RNA gene silencing through its interaction with histone H3 lysine 9 trimethylation and heterochromatin protein HP1-alpha. PMID: 26157143
- This research shows that lower-resolution mass spectrometry instruments can be utilized for histone post-translational modifications (PTMs) analysis. PMID: 25325711
- Inhibition of lysine-specific demethylase 1 activity prevented IL-1beta-induced histone H3 lysine 9 (H3K9) demethylation at microsomal prostaglandin E synthase 1 (mPGES-1) promoter. PMID: 24886859
- The authors report that de novo CENP-A assembly and kinetochore formation on human centromeric alphoid DNA arrays is regulated by a histone H3K9 acetyl/methyl balance. PMID: 22473132
