Acetyl-HIST1H4A (K16) Antibody
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Description
Target Specificity and Mechanism
The antibody targets HIST1H4A, a variant of histone H4, with acetylation at lysine 16. Acetylation at this site is catalyzed by histone acetyltransferases (HATs) and is linked to:
Key Features:
Applications and Validated Uses
This antibody is employed in diverse experimental workflows to study histone acetylation dynamics:
Common Applications
Dilution Recommendations
| Method | Suggested Dilution Range | Source |
|---|---|---|
| WB | 1:200–1:2000 | |
| IF/ICC | 1:20–1:200 | |
| ChIP | 5 µg per 5–10 µg chromatin | |
| IHC | 1:100–1:5000 |
Antibody Characteristics
Key Studies
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Cancer Epigenetics:
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Chromatin Dynamics:
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Drug Response:
Validation Data
Product Specs
Buffer
Preservative: 0.03% ProClin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Product dispatch occurs within 1-3 business days of order receipt. Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Synonyms
dJ160A22.1 antibody; dJ160A22.2 antibody; dJ221C16.1 antibody; dJ221C16.9 antibody; FO108 antibody; H4 antibody; H4.k antibody; H4/a antibody; H4/b antibody; H4/c antibody; H4/d antibody; H4/e antibody; H4/g antibody; H4/h antibody; H4/I antibody; H4/j antibody; H4/k antibody; H4/m antibody; H4/n antibody; H4/p antibody; H4_HUMAN antibody; H4F2 antibody; H4F2iii antibody; H4F2iv antibody; H4FA antibody; H4FB antibody; H4FC antibody; H4FD antibody; H4FE antibody; H4FG antibody; H4FH antibody; H4FI antibody; H4FJ antibody; H4FK antibody; H4FM antibody; H4FN antibody; H4M antibody; HIST1H4A antibody; HIST1H4B antibody; HIST1H4C antibody; HIST1H4D antibody; HIST1H4E antibody; HIST1H4F antibody; HIST1H4H antibody; HIST1H4I antibody; HIST1H4J antibody; HIST1H4K antibody; HIST1H4L antibody; HIST2H4 antibody; HIST2H4A antibody; Hist4h4 antibody; Histone 1 H4a antibody; Histone 1 H4b antibody; Histone 1 H4c antibody; Histone 1 H4d antibody; Histone 1 H4e antibody; Histone 1 H4f antibody; Histone 1 H4h antibody; Histone 1 H4i antibody; Histone 1 H4j antibody; Histone 1 H4k antibody; Histone 1 H4l antibody; Histone 2 H4a antibody; histone 4 H4 antibody; Histone H4 antibody; MGC24116 antibody
Target Names
HIST1H4A
Uniprot No.
Target Background
Function
Histone H4 (HIST1H4A) is a core component of the nucleosome, the fundamental structural unit of chromatin. Nucleosomes package and compact DNA, regulating access for cellular machinery involved in transcription, DNA repair, replication, and maintaining chromosomal stability. This accessibility is tightly controlled through a complex interplay of post-translational histone modifications, often referred to as the histone code, and dynamic nucleosome remodeling.
Gene References Into Functions
The following studies highlight the significant roles of histone H4 modifications in various cellular processes:
- PP32 and SET/TAF-Ibeta proteins inhibit HAT1-mediated H4 acetylation. PMID: 28977641
- H3K36me3 and H4K16ac are involved in DNA damage repair, with H3K36me3 stimulating H4K16ac following double-strand breaks. SETD2, LEDGF, and KAT5 are essential for these epigenetic changes. PMID: 28546430
- Omomyc protein co-localizes with c-Myc, PRMT5, and H4R3me2s-enriched chromatin domains. PMID: 26563484
- H4K12ac is regulated by estrogen receptor-alpha and is linked to BRD4 function and inducible transcription. PMID: 25788266
- Systemic lupus erythematosus is associated with an imbalance in histone acetyltransferases and deacetylases, leading to pathological H4 acetylation. PMID: 25611806
- Sumoylated histone H4 inhibits chromatin compaction by preventing long-range internucleosomal interactions. PMID: 25294883
- H4K5 acetylation is associated with lytic gene promoters during Kaposi's sarcoma-associated herpesvirus reactivation. PMID: 25283865
- Hypoxia-induced increases in histone H4 acetylation in neuroblastoma cells correlate with elevated N-myc transcription. PMID: 24481548
- G1-phase histone assembly is limited to CENP-A and H4. PMID: 23363600
- H4K12ac distribution in human sperm and its enrichment at specific promoter sites across the genome have been characterized. PMID: 22894908
- SRP68/72 heterodimers bind the histone H4 tail; this binding is inhibited by H4R3 methylation. PMID: 23048028
- TNF-alpha inhibits AQP5 expression in salivary gland cells via epigenetic suppression of histone H4 acetylation. PMID: 21973049
- Global histone H3 and H4 modification patterns are potential markers for tumor recurrence and survival in non-small cell lung cancer. PMID: 22360506
- HAT1 differentially affects nucleosome assembly of H3.1-H4 and H3.3-H4. PMID: 22228774
- PAK2-catalyzed phosphorylation of histone H4 Ser 47 promotes H3.3-H4 nucleosome assembly and inhibits H3.1-H4 assembly. PMID: 21724829
- Imatinib-induced hemoglobinization and erythroid differentiation in K562 cells are associated with global histone H4 modifications. PMID: 20949922
- Specific gene body sequences nucleate histone H4 lysine 20 monomethylation, reducing gene expression. PMID: 20512922
- Histone H4 expression is downregulated by zinc and upregulated by docosahexaenoate in a neuroblastoma cell line. PMID: 19747413
- Low histone acetylation is associated with gastric carcinoma development and progression, possibly through altered gene expression. PMID: 12385581
- MTA1 protein overexpression and histone H4 acetylation levels are closely correlated. PMID: 15095300
- PAD4 regulates histone Arg methylation by converting methyl-Arg to citrulline, impacting gene expression. PMID: 15345777
- Lack of histone H4K12 biotinylation is an early response to double-strand breaks. PMID: 16177192
- Acetylated H4K16 incorporation into nucleosomes inhibits the formation of compact chromatin fibers. PMID: 16469925
- Apoptosis is linked to global DNA hypomethylation and histone deacetylation in leukemia cells. PMID: 16531610
- BTG2 promotes retinoic acid-induced differentiation by modifying H4 arginine methylation and acetylation. PMID: 16782888
- Histone H4 modification, BDNF gene expression, and long-term memory for fear extinction are interrelated. PMID: 17522015
- The H4 tail and its acetylation mediate recruitment of regulatory factors influencing chromatin states and transcription. PMID: 17548343
- Brd2 bromodomain 2 interacts dynamically with H4-AcK12. PMID: 17848202
- Spermatid Hypac-H4 impairment in mixed atrophy is not worsened by AZFc region deletion. PMID: 18001726
- SET8 and PCNA interaction links H4-K20 methylation with DNA replication. PMID: 18319261
- H4K20 monomethylation and PR-SET7 are crucial for L3MBTL1 function. PMID: 18408754
- High acetylated H4 expression is more prevalent in aggressive cutaneous T-cell lymphoma. PMID: 18671804
- Histone H4 modifications play a significant role in bronchial carcinogenesis. PMID: 18974389
- H4K16 acetylation during S-phase results in an epigenetic label that persists through mitosis. PMID: 19348949
- Acetylated H4 is overexpressed in diffuse large B-cell and peripheral T-cell lymphomas. PMID: 19438744
- Sebaceous gland secretion of histone H4 may contribute to innate immunity. PMID: 19536143
- Histone modifications, including H3K27me3 and acH4, are involved in CD11b transcription during HL-60 cell differentiation. PMID: 19578722
- Cdk7 inhibition enhances histone H4 acetylation and diminishes H4K36me3, suggesting a role in regulating elongation. PMID: 19667075
- Histone H4 acetylation levels dynamically fluctuate during mitosis and respond to histone deacetylase inhibitors. PMID: 19805290
- BBAP is implicated in histone H4 monoubiquitylation and other modifications in the DNA damage response. PMID: 19818714
Database Links
Involvement In Disease
Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6.
Protein Families
Histone H4 family
Subcellular Location
Nucleus. Chromosome.
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