MTACP1 Antibody

Definition and Target Overview

mtACP1 is a conserved mitochondrial protein encoded by nuclear DNA. It serves as a scaffold for fatty acid synthesis in mitochondria, facilitating the production of octanoate—a precursor for lipoic acid, a cofactor essential for metabolic enzymes like pyruvate dehydrogenase . mtACP1 antibodies are tools to investigate its expression patterns, interaction networks, and roles in cell proliferation, redox regulation, and developmental biology .

Key Applications of mtACP1 Antibodies

mtACP1 antibodies are used in diverse experimental setups:

3.1. Role in Mitochondrial Fatty Acid Synthesis

• mtACP1 is required for synthesizing lipoic acid, which is critical for mitochondrial enzyme function (e.g., glycine decarboxylase) .

• In Arabidopsis, triple mtacp1/mtacp2/mtacp3 mutants are embryo-lethal, underscoring functional redundancy among isoforms .

3.2. Cell-Cycle and ROS Regulation

• Drosophila lacking mtACP1 exhibit reduced cell proliferation, elevated ROS, and mitochondrial complex I dysfunction .

• ROS scavengers (e.g., catalase) rescue growth defects in mtacp1 mutants, confirming ROS-mediated pathology .

3.3. Therapeutic Implications

• mtACP1 knockdown in cancer cells disrupts glycolysis and oxidative phosphorylation, suggesting metabolic vulnerabilities .

Protocol Considerations

• Fixation: Paraffin-embedded tissues require antigen retrieval (e.g., citrate buffer pH 6.0) .

• Dilution: Optimize concentrations (e.g., 1:200 for IF, 1:1000 for WB) .

Future Directions

Current research focuses on:

1. mtACP1’s role in cancer metabolism and immunotherapy resistance .

2. Engineering isoform-specific antibodies to dissect redundant functions in mammals .