AC85C Antibody
Description
Introduction to AC85C Antibody
The AC85C antibody is a polyclonal antibody specific to the AC85C protein derived from Solanum tuberosum (potato). It is primarily utilized in research applications such as enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) for detecting AC85C in plant samples. This antibody is produced by immunizing rabbits with recombinant AC85C protein and is affinity-purified to ensure specificity .
Key Properties of AC85C Antibody
| Property | Details |
|---|---|
| Target | AC85C protein (Solanum tuberosum) |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant AC85C protein |
| Reactivity | Confirmed for Solanum tuberosum |
| Applications | ELISA, Western blot (under reducing conditions) |
| Storage | -20°C or -80°C; avoid repeated freeze-thaw cycles |
| Conjugate | Non-conjugated |
| Purification Method | Antigen affinity-purified |
| Isotype | IgG |
| Lead Time | 14–16 weeks (custom production) |
Research Applications
The AC85C antibody is designed for research use only, with applications including:
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Protein Detection: Identifies AC85C in potato tissue lysates via Western blot, producing bands at predicted (~50 kDa) and observed (~72 kDa) molecular weights .
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ELISA: Quantifies AC85C levels in experimental setups, though specific validation data are not publicly disclosed.
Limitations and Considerations
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Species Specificity: Reactivity is confirmed only for Solanum tuberosum. Cross-reactivity with other plant species has not been reported.
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Experimental Validation: Peer-reviewed studies or functional data (e.g., epitope mapping, binding kinetics) are absent in the literature.
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Commercial Availability: Distributed exclusively by Cusabio under product code CSB-PA335522XA01FIG .
Comparative Analysis with Related Antibodies
While AC85C itself is not widely studied, research on structurally similar antibodies provides context:
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Ag85 Complex Antibodies: Antibodies targeting the mycobacterial Ag85 complex (e.g., Ag85A, Ag85B) demonstrate high specificity and utility in diagnostics and vaccine development . These studies highlight the importance of epitope characterization, which remains unexplored for AC85C.
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Antibody Engineering: Techniques such as glycoengineering (e.g., afucosylation) enhance antibody effector functions , but their applicability to plant-derived antibodies like AC85C is unknown.
Future Directions
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Epitope Mapping: Structural studies (e.g., X-ray crystallography) are needed to define AC85C’s binding sites.
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Functional Studies: Investigations into AC85C’s role in potato physiology or stress responses could expand its research relevance.
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Cross-Species Reactivity: Screening for reactivity in related plant species (e.g., Solanum lycopersicum) may broaden utility.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
