ACT9 Antibody

ACOT9 Antibody Overview

ACOT9 antibodies are immunoreagents designed to detect and quantify the ACOT9 protein in experimental settings. These antibodies are critical for studying ACOT9's role in cellular metabolism, mitochondrial function, and disease associations. Commercial ACOT9 antibodies are typically affinity-purified polyclonal or monoclonal antibodies validated for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) .

Research Applications

ACOT9 antibodies are primarily used to:

• Map protein expression in human tissues (e.g., liver, kidney, and heart) via IHC .

• Investigate mitochondrial dysfunction in metabolic disorders like obesity or diabetes.

• Validate ACOT9 knockdown/overexpression in cell-based assays using Western blotting.

Example Workflow for ACOT9 Detection:

1. Tissue fixation: Paraffin-embedded sections treated for antigen retrieval.

2. Primary antibody incubation: Diluted ACOT9 antibody applied (1:1000–1:2500).

3. Signal detection: HRP-conjugated secondary antibody and chromogenic substrate.

Validation and Quality Control

Commercial ACOT9 antibodies undergo rigorous validation:

• Specificity: Verified using siRNA-mediated ACOT9 knockdown and overexpression models.

• Reproducibility: Batch-to-blot consistency confirmed across multiple lots .

• Cross-reactivity: No observed reactivity with unrelated thioesterases (e.g., ACOT1, ACOT2).