anti-Homo sapiens (Human) ACTC1/ACTA1/ACTG2 Monoclonal Antibody raised in Mouse

Description

Introduction to ACTC1/ACTA1/ACTG2 Monoclonal Antibody

The ACTC1/ACTA1/ACTG2 Monoclonal Antibody is a highly specific immunoglobulin targeting three actin isoforms: ACTC1 (actin alpha cardiac muscle 1), ACTA1 (actin alpha skeletal muscle), and ACTG2 (actin gamma-2 smooth muscle). These isoforms are critical for cellular motility, muscle contraction, and structural integrity . Monoclonal antibodies (mAbs) like this are derived from a single cell line, ensuring uniform specificity and reproducibility . This antibody is widely used in research to study actin-related pathologies, including cardiomyopathies, myopathies, and cancer metastasis .

ACTC1 (Cardiac Muscle Actin)

Function: Essential for cardiac muscle contraction and sarcomere organization .
Clinical Relevance: Mutations linked to dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM) .

ACTA1 (Skeletal Muscle Actin)

Function: Dominant isoform in adult skeletal muscle; mediates force generation during contraction .
Clinical Relevance: Mutations associated with nemaline myopathy and congenital myopathies .

ACTG2 (Smooth Muscle Gamma-2 Actin)

Function: Critical for smooth muscle contraction in vascular and gastrointestinal tissues .
Clinical Relevance: Dysregulation implicated in glioblastoma migration and visceral myopathies .

Antibody Characteristics

The ACTC1/ACTA1/ACTG2 Monoclonal Antibody (e.g., clone ABT-ACTN) exhibits the following properties :

Property	Details
Host Species	Mouse
Isotype	IgG
Reactivity	Human, Mouse, Rat
Applications	Western Blot (WB), Immunohistochemistry (IHC-Paraffin)
Molecular Weight Detected	42 kDa
Epitope Specificity	Acetylated N-terminal regions (critical for isoform specificity)
Storage Conditions	-20°C; stable for 12 months
Purification Method	Immunogen affinity purified

Diagnostic and Mechanistic Studies

Cardiac Research: Used to detect ACTC1 expression in myocardial tissue, aiding in the diagnosis of cardiomyopathies .
Cancer Biology: ACTG2 overexpression in glioblastoma correlates with tumor invasiveness; this antibody helps identify metastatic potential .
Muscle Disorders: Enables differentiation between ACTA1-related myopathies and other muscular dystrophies .

Functional Insights

Contractility Studies: Acta2 (smooth muscle actin) knockout mice showed defective lactation due to impaired myoepithelial cell contraction, highlighting actin isoform specificity .
Cytoskeletal Dynamics: In glioblastoma cells, ACTA2 knockdown reduced migration and proliferation, validated using this antibody .

Comparative Data from Key Studies

Study	Key Finding	Antibody Utility
Skalli et al. (1986)	Acta2 deficiency disrupts mammary gland contractility	Confirmed ACTA2’s role in myoepithelial function
TCGA Database Analysis	High ACTA2 expression linked to shorter progression-free survival in gliomas	Identified ACTA2 as a prognostic marker
Proteintech Validation	ACTA1-specific antibody does not cross-react with cytoplasmic actins	Demonstrated isoform specificity in skeletal muscle

Product Specs

Buffer

The antibody is provided as a liquid solution in phosphate-buffered saline (PBS) containing 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide.

Form

Liquid

Lead Time

Typically, we are able to ship products within 1-3 business days of receiving your order. Delivery times may vary depending on the chosen shipping method and destination. For specific delivery estimates, we recommend contacting your local distributor.

Uniprot No.

P68032 P68133 P63267

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ACTC1/ACTA1/ACTG2 Monoclonal Antibody