ADCY7 Antibody
Description
Definition and Background
ADCY7 Antibody is a polyclonal immunoglobulin produced in rabbits against a synthesized peptide derived from the human ADCY7 protein (amino acids 191–240) . It specifically recognizes ADCY7, a calcium-inhibitable enzyme belonging to the adenylyl cyclase class-4/guanylyl cyclase family, which regulates intracellular cAMP levels .
Immune System Regulation
ADCY7 is critical for modulating innate and adaptive immunity. Studies using ADCY7-deficient mice revealed its role in:
The ADCY7 antibody facilitated the identification of reduced lymphocyte counts in chimeric mice, highlighting its importance in hematopoiesis .
Cancer Biology
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Acute Myeloid Leukemia (AML): ADCY7 supports leukemia cell survival by suppressing apoptosis and maintaining c-Myc expression . Knockdown experiments using ADCY7-targeting tools reduced cell growth by 30–40% in AML lines .
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Hepatocellular Carcinoma (HCC): ADCY7 promotes CD8+ T-cell infiltration via nuclear translocation and CCL5 transcription, enhancing antitumor immunity . Exosomal ADCY7 reshapes the tumor microenvironment, making it a potential therapeutic target .
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Cervical Cancer: High ADCY7 expression correlates with poor prognosis and immune cell infiltration (e.g., macrophages, neutrophils) .
Neurological Disorders
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ADCY7 transcript levels are elevated in the amygdala of individuals with major depressive disorder (MDD) and serotonin transporter knockout mice .
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The SNP rs1064448 in ADCY7 influences threat-related amygdala reactivity, linking it to emotional regulation .
Signaling Pathways
ADCY7 modulates cAMP synthesis, which impacts:
Supplier and Validation Data
The ADCY7 antibody (Catalog #A11384) is commercially available from Boster Bio . Validation includes:
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Specificity confirmed via Western blot, IHC, and ELISA across human and mouse samples .
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Compatibility with multiple experimental platforms, including fluorescence-based assays .
Limitations and Future Directions
While ADCY7 antibodies have proven invaluable, challenges remain:
Product Specs
Target Background
ADCY7 is an enzyme that catalyzes the formation of cyclic adenosine monophosphate (cAMP) in response to G protein-coupled receptor activation. It plays a crucial role in various signaling cascades, including those triggered by thrombin and sphingosine 1-phosphate. ADCY7 regulates cAMP synthesis synergistically with stimulatory Gα proteins (GNA13 and Gβγ complexes), influencing pathways activated by agonists such as thrombin, sphingosine 1-phosphate, dopamine, and the C5 alpha chain. During inflammation, ADCY7 mediates zymosan-induced cAMP increases, activating protein kinase A and modulating innate immune responses via heterotrimeric G(12/13) proteins. Furthermore, ADCY7 regulates inflammation during bacterial infection by responding to serum factors like lysophosphatidic acid (LPA), influencing LPS-induced TNF-alpha production. Importantly, it is also essential for optimal B and T cell function in adaptive immunity by regulating cAMP synthesis in these cell types.
The functional significance of ADCY7 is underscored by numerous studies:
- A 0.6% frequency missense variant in ADCY7 has been identified. (PMID: 28067910)
- Sex-specific differences in TNF-alpha responses may be partially attributed to male-specific downregulation of ADCY7 and ADCY9. (PMID: 25959651)
- ADCY7 deficiency has been linked to decreased cell growth, increased apoptosis, and reduced c-Myc expression in acute myeloid leukemia cells. (PMID: 26220344)
- Evidence suggests a role for ADCY7 in modulating mood regulation and potentially contributing to depression risk and pathophysiology. (PMID: 22264442)
- Single nucleotide polymorphisms in ADCY7 have been associated with alcohol dependence in women and correlate with ADCY7 mRNA expression levels. (PMID: 21481845)
- Studies have detailed the construction of soluble adenylyl cyclase from human membrane-bound type 7 adenylyl cyclase. (PMID: 11665607)
- The first cytoplasmic domain (residues 506-584) of ADCY7 acts as an internal regulatory subunit, interacting with Gsα and the first catalytic domain. (PMID: 15581358)
- Brain-specific ADCY7 isoforms exhibit a sex-specific role in heritable depressive symptoms in genetically modified mice. (PMID: 17135423)
- ADCY7 serves as a specific downstream effector of the G(12/13) pathway. (PMID: 18541530)
- Soluble adenylyl cyclase responds to both CO2 and bicarbonate ions. (PMID: 19008230)
