ADH1 Antibody

Introduction to ADH1 Antibody

ADH1 (Alcohol Dehydrogenase 1) antibodies are immunological tools designed to detect and study class I alcohol dehydrogenases, enzymes critical for ethanol metabolism and retinoid processing . These antibodies are widely used in research to investigate ADH1's roles in metabolic pathways, adipogenesis, and pathogen biology . ADH1 exists in isoforms (ADH1A, ADH1B, ADH1C) encoded by distinct genes, with ADH1B being primate-specific and highly expressed in adipose tissue and liver .

Functional Roles

Antibody Production

• Immunogens: Full-length ADH1 isolated from yeast , recombinant human/mouse ADH1 .

• Host Systems: Rabbit (polyclonal) , mouse (monoclonal) .

Cross-Reactivity and Validation

Key Findings

1. Adipocyte Differentiation:

   • CRISPR knockout of ADH1B in human adipose stem cells blocked lipid accumulation and adipogenic markers (PPARγ, C/EBPα) .

   • Rescue experiments confirmed 9-cis retinoic acid (not retinol) restores differentiation, highlighting ADH1B’s role in RA synthesis .

2. Pathogen Studies:

   • Anti-Candida Adh1 monoclonal antibody (Ca37) reduced fungal virulence in Galleria mellonella models .

3. Metabolic Regulation:

   • Insulin upregulates ADH1B in adipocytes via AKT signaling, enhancing glucose uptake .

Selected ADH1 Antibodies

Performance Notes

• Western Blot: Detects ~37 kDa (yeast) or ~40 kDa (human/mouse) bands .

• Immunofluorescence: Requires cell wall pretreatment (e.g., sodium metaperiodate) for fungal epitope access .

Implications in Metabolic and Pathogenic Research

• Obesity and Diabetes: Reduced ADH1B expression in obesity correlates with impaired adipogenesis and insulin resistance .

• Alcohol Dependence: ADH1B polymorphisms (e.g., Arg313Cys) alter enzymatic activity and influence alcoholism risk .

• Antifungal Therapy: Anti-Adh1 antibodies show potential for targeting Candida without cross-reacting with human proteins .