ADK1 Antibody

Applications in Research and Diagnostics

ADK1 antibodies are utilized across multiple experimental workflows:

Table 1: Primary Applications and Performance

Comparative Binding Affinities

ADK1a exhibits high specificity for AAV1, with binding affinities (KD) significantly stronger than its human chimeric derivative (ADK1a-h1):

Table 2: Binding Affinities Across Serotypes

Source: Biolayer interferometry (BLI) data

The human chimeric variant ADK1a-h1 retains neutralizing activity but shows reduced affinity, making it suitable for serology studies requiring humanized components .

Mechanistic Insights from Neutralization Studies

ADK1a effectively inhibits AAV1 and AAV6 transduction by occluding capsid regions essential for cellular entry. In luciferase-based neutralization assays:

• Preincubation of ADK1a (2 ng/ml) with AAV1-NanoLuc particles reduces luminescence signal by 50% in HEK293 cells .

• Epitope stability under physiological conditions ensures robust neutralization across diverse AAV variants .