At1g06350 Antibody

Gene Identification and Protein Function

AT1G06350 is part of the acyl-lipid desaturase (ADS) family in Arabidopsis, which includes ADS1, ADS2, and ADS3 . These enzymes introduce double bonds into fatty acids esterified to membrane lipids, a process critical for maintaining membrane fluidity under stress conditions like chilling or freezing .

Key Features of ADS4 (AT1G06350):

• Function: Predicted Δ9 acyl-lipid desaturase involved in lipid remodeling during stress responses .

• Domain Structure: Contains conserved histidine-rich motifs typical of desaturases, enabling iron-dependent catalytic activity .

• Subcellular Localization: Likely localized to plastids or endoplasmic reticulum, based on homology to ADS2 and ADS3 .

Antibody Applications in Research

Antibodies against ADS4 would enable precise detection and localization of the protein. While no direct studies on the ADS4 antibody are cited, analogous research on related desaturases and antibodies provides insight into its potential uses:

Table 1: Potential Applications of the At1g06350 Antibody

Stress Response and Lipid Metabolism

• Chilling Tolerance: ADS2 (a homolog) is essential for acclimation to low temperatures by increasing unsaturated lipid content . ADS4 likely plays a complementary role.

• DNA Damage Response: AT1G06350 is identified as a hub gene in Arabidopsis subnetworks responding to low-dose radiation, implicating it in stress signaling .

Table 2: Network Analysis of AT1G06350 in Stress Responses

Technical Considerations for Antibody Development

• Epitope Specificity: Engineered monoclonal antibodies (mAbs) require precise alignment of complementarity-determining regions (CDRs) for target binding .

• Validation: Cross-reactivity with other ADS family members (e.g., ADS2) must be ruled out via knockout controls .

• Therapeutic Potential: While ADS4 itself is not a clinical target, antibody engineering methods (e.g., pH-dependent binding ) used in disease models could inform plant biology tools.

Future Directions

• Functional Studies: Use the antibody to characterize ADS4’s role in lipid rafts or stress granule formation.

• Comparative Analysis: Compare ADS4 expression in wild-type vs. ads4 mutants under abiotic stress .

• Structural Biology: Solve ADS4’s crystal structure to guide antibody optimization for higher affinity .