At1g06360 Antibody

Target Protein: At1g06360

The At1g06360 gene encodes a LEM3 (ligand-effect modulator 3) family protein, also classified under the CDC50 family of membrane transporters . These proteins are implicated in lipid flipping and cellular membrane homeostasis. Additionally, At1g06360 has been annotated as a fatty acid desaturase family protein, suggesting potential roles in lipid metabolism and stress adaptation .

Key Features of the At1g06360 Protein:

A. Functional Insights

• Membrane Lipid Dynamics: Proteins in the CDC50/LEM3 family, like At1g06360, are critical for lipid asymmetry maintenance and vesicular trafficking. Homologs in other species interact with P4-ATPases to facilitate phospholipid translocation .

• Stress Adaptation: Fatty acid desaturases modulate membrane fluidity under abiotic stress (e.g., chilling). While At1g06360’s direct role remains unconfirmed, related genes (e.g., ADS2) are essential for cold tolerance in Arabidopsis .

Potential Research Applications

The At1g06360 antibody could be utilized in:

1. Protein Localization Studies: Mapping subcellular distribution via immunofluorescence or GFP fusion assays.

2. Expression Profiling: Quantifying protein levels under stress conditions (e.g., cold, drought) using Western blotting.

3. Interaction Networks: Identifying binding partners through co-immunoprecipitation (Co-IP) and mass spectrometry.

Comparative Analysis of Related Proteins

Limitations and Future Directions

• Functional Data Gap: No peer-reviewed studies directly using the At1g06360 antibody are available, necessitating validation experiments.

• Technical Challenges: Antibody specificity and cross-reactivity with related CDC50 family members require verification.

• Opportunities: CRISPR/Cas9-generated at1g06360 mutants could elucidate its role in lipid signaling or stress responses .