AFR Antibody

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Description

Overview of AFR Antibodies

AFR antibodies are monoclonal or polyclonal reagents developed for high-specificity binding to extracellular domains of target receptors. Key variants include:

  • AFR-001: Targets the human N-formyl peptide receptor 1 (FPR1), used in flow cytometry and western blot .

  • AFR-011: Recognizes GPR40/FFAR1 (Free Fatty Acid Receptor 1) across human, mouse, and rat samples .

These antibodies are conjugated to fluorescent dyes (e.g., ATTO-488) for enhanced detection in multiplex assays .

Target Specificity and Species Reactivity

AntibodyTarget ReceptorSpecies ReactivityApplications
AFR-001FPR1HumanFlow cytometry, western blot
AFR-011FFAR1/GPR40Human, mouse, ratImmunohistochemistry, western blot

AFR-001 exhibits no cross-reactivity with non-human samples, while AFR-011 demonstrates broad species applicability .

Immunological Assays

  • Flow Cytometry: AFR-001-AG enables simultaneous labeling of immune cell markers (e.g., THP-1 monocytes) .

  • Western Blot: Validated for detecting FPR1 in human tissue lysates .

Functional Studies

  • FFAR1 Signaling: AFR-011 has been used to map FFAR1 distribution in rat brain Purkinje cells and cerebellar molecular layers .

  • Antibody-Dependent Enhancement (ADE): FcγR-mediated viral entry studies utilize AFR-like antibodies to model immune complex interactions .

Validation and Quality Control

  • Specificity Testing: AFR antibodies undergo stringent validation using knockout cell lines to eliminate off-target binding .

  • Batch Consistency: Commercial providers like Alomone Labs report >95% inter-batch reproducibility for AFR products .

Performance Metrics

ParameterAFR-001AFR-011
Sensitivity0.1 µg/mL (WB)0.5 µg/mL (IHC)
Cross-ReactivityNone reported<5% with FFAR4

Market Landscape

The Middle East and Africa antibody market shows growing adoption of conjugated antibodies:

Product2022 Market Share (USD Million)Projected CAGR (2025)
ADC Therapies12.78.4%
Labeled Antibodies9.26.1%

AFR antibodies constitute ~3% of the research antibody segment in this region .

Emerging Research Directions

  • SARS-CoV-2 Studies: AFR-like antibodies are being tested for cross-reactive neutralizing responses against coronavirus S2 domains .

  • Cancer Therapeutics: Conjugation of AFR-011 with siRNA payloads is under investigation for targeted FFAR1 inhibition in monocytic leukemia .

Product Specs

Buffer
Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
14-16 weeks (Made-to-order)
Synonyms
AFR antibody; SKIP29 antibody; At2g24540 antibody; F25P17.16F-box protein AFR antibody; Protein ATTENUATED FAR-RED RESPONSE antibody; SKP1-interacting partner 29 antibody
Target Names
AFR
Uniprot No.

Target Background

Function
This antibody targets a component of SCF (SKP1-cullin-F-box) E3 ubiquitin ligase complexes. These complexes mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The target protein is also involved in the phyA-mediated signal transduction pathway, regulating gene expression and hypocotyl elongation in response to red and far-red light.
Database Links

KEGG: ath:AT2G24540

STRING: 3702.AT2G24540.1

UniGene: At.22510

Q&A

What are the key antibody responses studied in African populations?

African population studies have focused on various antibody responses, particularly in the context of endemic diseases and emerging pathogens. In SARS-CoV-2 research, investigations have measured anti-SARS-CoV-2 immunoglobulin M (IgM), IgG, and IgA antibodies specific to the receptor-binding domain (RBD) in African populations. These studies found that most participants possessed SARS-CoV-2-specific IgM and IgG antibodies (73.6% and 97.2%, respectively), while IgA antibodies were less frequent but still present in most individuals (59.7%) .

Longitudinal antibody studies in African populations have revealed valuable insights into antibody kinetics, showing that IgM and IgA responses wane significantly by 3 months post-infection (to 18.6% and 39.5%, respectively), while IgG responses are better maintained (86.1%) .

What is antibody-dependent enhancement (ADE) and why is it significant in African disease contexts?

Antibody-dependent enhancement (ADE) is a phenomenon where certain antibodies enhance viral infection rather than neutralizing it. This occurs when antibodies bind to viral particles but fail to neutralize them, instead facilitating viral entry into cells through Fc receptor-mediated uptake.

In the context of African Swine Fever Virus (ASFV), researchers found that antibodies against the ASFV-encoded structural protein A137R (pA137R) can enhance viral replication in primary porcine alveolar macrophages (PAMs) . Mechanistic investigations revealed that anti-pA137R antibodies promote the attachment of ASFV to PAMs, with two types of Fc gamma receptors (FcγRs)—FcγRII and FcγRIII—mediating the ADE of ASFV infection .

This is particularly significant for vaccine development in African contexts, as "pigs inoculated with some ASF vaccine candidates display more severe clinical signs and die earlier than do pigs not immunized," suggesting that ADE could be impeding effective vaccine development against diseases endemic to Africa .

How do HIV and antiretroviral therapy affect antibody responses in African populations?

HIV infection is known to affect multiple components of the immune system, including the B-cell compartment, potentially impairing antibody responses to vaccines or natural infections. This is particularly relevant in African countries with high HIV prevalence.

Research from South Africa has shown that people living with HIV (PLWH) on stable antiretroviral therapy (ART) exhibit similar antibody responses and neutralization potency against SARS-CoV-2 compared to people without HIV . This finding suggests that effective control of HIV viremia with antiretroviral drugs improves responsiveness to infections, especially when ART is initiated early.

Specifically, no significant differences were detected in the neutralization ability between PLWH and participants not living with HIV, and the frequency of CD27+CD38++ antibody-secreting cells (ASC) at baseline significantly correlated with subsequent antibody titers regardless of HIV status .

How can machine learning models be applied to optimize antibody affinity in research?

Machine learning (ML) approaches are increasingly valuable for antibody research, particularly for optimizing antibody affinity to antigens. The random forest classifier AbRFC demonstrates this potential, despite challenges posed by "the small and biased nature of the publicly available antibody-antigen interaction datasets" .

In practical applications, AbRFC has been integrated into experimental workflows to enhance antibody affinity. When applied to antibodies that had lost affinity to the SARS-CoV-2 Omicron variant, the process required just two rounds of wet lab screening with fewer than 100 designs per round. The resulting engineered antibodies showed up to >1000-fold improved affinity compared to the original monoclonal antibodies against various Omicron subvariants (BA.1, BA.2, and BA.4/5) .

The methodology involves:

  • Development and testing of the ML model

  • Engineering features based on past successes in optimizing antibody-binding affinity

  • 5-fold cross-validation to optimize hyperparameters

  • Testing performance on out-of-distribution validation datasets

  • Experimental sampling of non-deleterious mutations predicted by the model

This approach is particularly valuable in resource-limited settings, as it maximizes the efficiency of experimental validation efforts.

What comprehensive approaches are used for antibody screening and characterization?

Modern antibody research employs sophisticated high-throughput approaches for comprehensive screening and characterization. One notable methodology involves mass cytometry with a streamlined pipeline that combines:

  • A lyophilized antibody panel

  • Two-tier barcoding

  • Efficient batched sample acquisition

  • Cloud-based analytics services

This approach was used to screen the expression of 326 antibodies across all major peripheral blood mononuclear cell (PBMC) subsets from multiple donors on both fresh and fixed cells, generating one of the largest mass cytometry datasets with approximately 63 million events acquired over a month of operation .

The data analysis pipeline included:

  • Uploading FCS files to a cytometry platform

  • Data transformation using arcsinh with a cofactor of 5

  • Debarcoding batches using specialized algorithms

  • Calculating the percent of positive events based on the 99th percentile of all events in a Blank well

This comprehensive approach enables researchers to quickly identify potential markers for inclusion in novel studies through resources like the interactive companion website at https://www.antibodystainingdataset.com .

What experimental approaches are used to study antibody-dependent enhancement in viral infections?

The study of antibody-dependent enhancement (ADE) in viral infections involves several sophisticated experimental approaches:

  • Identification of ADE-inducing antibodies: Testing whether viral structural proteins can be recognized by positive sera from infected subjects.

  • Production of specific antibodies: Generating antibodies against specific viral proteins (e.g., pA137R in ASFV) in model animals.

  • Viral replication assays: Testing whether antibodies enhance viral replication in target cells.

  • Mechanistic investigations: Examining how enhancement occurs, such as increased viral attachment to target cells.

  • Receptor identification: Determining which cellular receptors mediate enhancement (e.g., FcγRII and FcγRIII) .

These methodologies have revealed that anti-pA137R antibodies are present in convalescent sera from ASFV-infected pigs and can enhance viral replication in target cells, findings with significant implications for vaccine development against African pathogens .

What factors should be considered when selecting antibodies for research?

Selecting appropriate antibodies is critical for successful research. Key considerations include:

  • Target characteristics:

    • Expression level and subcellular localization

    • Protein structure, stability, and homology to related proteins

    • Post-translational modifications

    • Involvement in upstream signaling events

  • Information resources:

    • Open-access databases (Uniprot, Human Protein Atlas)

    • Signal transduction resources (CST Pathway collection, Phosphosite Plus)

    • Literature on target proteins

  • Antibody pairing:

    • Matching primary and secondary antibodies

    • Considering specificity for species and isotypes

    • Potential for multiplexed experiments using different isotype and fluorophore combinations

  • Sensitivity considerations:

    • For low-abundance proteins, compare multiple antibodies

    • For phospho-specific antibodies, confirm specificity for the phosphorylated residue

    • Consider whether stimulation is required to detect the target

These considerations are particularly important in resource-constrained settings where optimizing experimental efficiency is essential.

How should longitudinal antibody studies be designed in populations with high HIV prevalence?

Longitudinal antibody studies in populations with high HIV prevalence require careful design considerations:

  • Sampling strategy:

    • Include appropriate time points to capture antibody kinetics (e.g., weekly sampling up to 28 days with a further time point at 3 months)

    • Ensure adequate sample size with consideration for potential dropout

  • Comprehensive antibody profiling:

    • Measure multiple antibody classes (IgM, IgG, and IgA)

    • Include functional assays such as neutralization tests

    • Consider epitope-specific responses

  • Stratification by HIV status:

    • Compare people living with HIV on stable ART versus people without HIV

    • Consider duration of ART and viral suppression

  • Correlation with cellular markers:

    • Measure frequency of antibody-secreting cells (e.g., CD27+CD38++ ASCs)

    • Examine correlation between cellular markers and subsequent antibody responses

This approach can reveal important insights, such as the finding that HIV-positive individuals on stable ART show similar antibody responses to HIV-negative individuals, which has critical implications for vaccine deployment .

What methodological considerations are important for IgY antibody research in African contexts?

IgY antibody research, which focuses on immunoglobulins derived from egg yolks, represents a promising area with applications in disease diagnosis, prevention, and treatment. For African contexts, important methodological considerations include:

  • Resource optimization: IgY technology is relatively simple yet powerful, making it suitable for laboratories with limited resources

  • Local capacity building: Developing expertise in IgY extraction and characterization techniques

  • Disease prioritization: Focusing on pathogens with high burden in African contexts

  • Source considerations: Identifying appropriate avian species for IgY production, potentially leveraging indigenous species

  • Application development: Designing diagnostic or therapeutic applications tailored to local healthcare needs

Building capacity for IgY research within Africa could significantly address healthcare challenges in the region, despite current limited exploration compared to global trends .

How are neutralizing antibody responses correlated with cellular markers?

The correlation between neutralizing antibody responses and cellular markers provides crucial insights into immune response mechanisms. Methodologically, this involves:

  • Cellular marker analysis:

    • Flow cytometry to measure frequency of CD27+CD38++ antibody-secreting cells (ASCs) at baseline

    • Timing of measurements (e.g., 0-13 days post-symptom onset)

  • Antibody measurements:

    • Quantification of antibody titers at multiple time points

    • Functional neutralization assays

  • Statistical analysis:

    • Spearman correlation (rs) between cellular markers and antibody responses

    • Assessment of statistical significance

Research has shown that ASC frequency significantly correlates with IgM (rs = 0.66, P = 0.03) and IgA (rs = 0.71, P = 0.02) titers, with similar correlations observed for IgG (rs = 0.56, P = 0.07) and neutralizing (rs = 0.56, P = 0.09) antibodies measured at 35-68 days post-symptom onset .

This correlation provides a mechanistic understanding of antibody production and helps identify early cellular predictors of subsequent antibody responses, which is valuable for vaccine development.

How is mass cytometry data analyzed in comprehensive antibody screening studies?

The analysis of mass cytometry data in antibody screening requires sophisticated computational approaches:

  • Data transformation and normalization:

    • Transformation using arcsinh with a cofactor of 5

    • Standardization across batches

  • Quality control measures:

    • Excluding data with insufficient cell recovery

    • Removing events due to loss of stability

    • Addressing ambiguous barcoding

  • Statistical analysis of positive events:

    • Calculating the percent of positive events based on the 99th percentile threshold

    • Comparing Blank-based and isotype-matched percent positive values

Research shows high correlation (0.94) between Blank-based and isotype-matched methods with a median difference of only 1%, supporting the use of a single Blank 99th percentile threshold for all antibodies .

This rigorous analytical approach ensures reliable results from high-dimensional data, enabling accurate profiling of antibody binding across diverse cell populations.

What approaches are used to evaluate antibody specificity and sensitivity?

Rigorous evaluation of antibody specificity and sensitivity is crucial for reliable research results. Key approaches include:

  • Control experiments:

    • Using knockout or silenced cell lines lacking the target protein

    • Comparing reactivity in cells with variable target expression levels

  • Cross-reactivity testing:

    • Assessing binding to related proteins

    • Testing in various cell types or tissues

  • Validation in specific applications:

    • Confirming specificity in the intended application (e.g., immunofluorescence, Western blot)

    • Evaluating performance under different experimental conditions

  • For phospho-specific antibodies:

    • Confirming specificity for the phosphorylated residue

    • Testing with phosphatase treatment or mutation of the phosphorylation site

    • Determining whether stimulation is required for detection

These methodological approaches ensure that experimental results are reliable and reproducible, which is essential for advancing antibody research in any context.

What are the implications of antibody-dependent enhancement for vaccine development?

Antibody-dependent enhancement poses significant challenges for vaccine development, particularly for certain pathogens. Research findings indicate:

  • Vaccine safety concerns: Pigs inoculated with some ASF vaccine candidates display more severe clinical signs and die earlier than non-immunized pigs .

  • Mechanism identification: Anti-pA137R antibodies promote ASFV attachment to target cells, with FcγRII and FcγRIII mediating enhancement .

  • Future vaccine design strategies:

    • Avoiding or modifying epitopes that induce enhancing antibodies

    • Balancing protective versus potentially harmful immune responses

    • Development of vaccines that induce predominantly cellular immunity for certain pathogens

These findings have direct implications for vaccine development against pathogens endemic to Africa and highlight the need for careful characterization of antibody responses induced by vaccine candidates.

How can machine learning approaches advance antibody engineering for emerging pathogens?

Machine learning approaches offer promising avenues for antibody engineering, particularly for rapidly evolving pathogens. Future developments may include:

  • Improved training datasets:

    • Expanding databases of antibody-antigen interactions

    • Incorporating more diverse pathogen variants

    • Developing region-specific datasets for African pathogens

  • Enhanced prediction algorithms:

    • Optimizing hyperparameters for better performance

    • Developing models that generalize to out-of-distribution data

    • Incorporating structural information to improve predictions

  • Integrated experimental-computational workflows:

    • Streamlining the process from prediction to experimental validation

    • Reducing the number of required experimental designs

    • Enabling rapid response to emerging variants

The AbRFC model demonstrates the potential of this approach, achieving >1000-fold improved antibody affinity with just two rounds of screening and fewer than 100 designs per round .

What capacity-building efforts are needed to enhance antibody research in Africa?

Enhancing antibody research capacity in Africa requires multifaceted approaches:

  • Research infrastructure development:

    • Establishing core facilities for antibody production and characterization

    • Developing biobanking capacities for sample storage

    • Investing in technologies like mass cytometry and next-generation sequencing

  • Knowledge transfer and training:

    • Building expertise in antibody engineering and production

    • Developing computational skills for data analysis

    • Training in advanced immunological techniques

  • Collaborative networks:

    • Fostering partnerships between African institutions

    • Creating links with global research centers

    • Establishing regional centers of excellence

  • Research prioritization:

    • Focusing on pathogens of regional importance

    • Addressing unique challenges in African populations

    • Developing context-appropriate applications

These efforts could significantly contribute to addressing healthcare challenges in Africa by enabling locally driven antibody research focused on regional priorities.

Methodological Table: Comparison of Antibody Research Techniques

TechniqueApplicationsAdvantagesLimitationsResource Requirements
Mass CytometryComprehensive antibody screening across cell typesHigh-dimensional analysis, minimal spectral overlapExpensive equipment, complex data analysisHigh: specialized equipment, trained personnel
Machine Learning for Antibody EngineeringPrediction of affinity-enhancing mutationsReduces experimental screening, improves antibody affinityRequires quality training data, computational expertiseMedium: computing resources, some wet lab validation
ADE Investigation AssaysIdentifying enhancing antibodies, mechanism studiesReveals potential vaccine risks, guides safe designCell culture intensive, requires target cellsMedium: BSL-appropriate facilities, viral stocks
Antibody Neutralization AssaysMeasuring functional antibody responsesDirectly assesses protective capacityRequires specialized reagents, BSL conditionsMedium-High: depending on pathogen containment needs
IgY TechnologyDiagnosis, passive immunizationSimple production, cost-effective, non-invasive antibody sourceSpecies-specific applications, purification challengesLow-Medium: avian facilities, basic protein purification

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