AGL80 Antibody
Description
Clarifying the Antibody’s Target
The Abcam product labeled "Anti-AGL/Alpha-glucosidase antibody [EPR8880]" (ab133720) appears to target Alpha-glucosidase, an enzyme involved in carbohydrate metabolism, rather than the Arabidopsis transcription factor AGL80 . This mislabeling creates ambiguity in research applications.
Experimental Data for Alpha-Glucosidase Antibody
The Abcam antibody (ab133720) has been tested in western blot assays across multiple species:
Western Blot Validation
| Sample Type | Species | Lane Contents | Antibody Dilution | Predicted Band |
|---|---|---|---|---|
| Whole cell lysates | Human (K-562) | 20 µg lysate | 1:1000 | 175 kDa |
| Whole cell lysates | Human (HEK-293) | 20 µg lysate | 1:1000 | 175 kDa |
| Tissue lysates | Human (fetal muscle, heart) | 20 µg lysate | 1:5000 | 175 kDa |
| Tissue lysates | Mouse, Rat (muscle) | 20 µg lysate | 1:5000 | 175 kDa |
Key Observations:
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The antibody detects a 175 kDa band in human and rodent samples, consistent with Alpha-glucosidase’s molecular weight .
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No data are available for plant species, where AGL80 (the transcription factor) is active.
AGL80 (Arabidopsis) and Its Role
AGL80 (AT5G48670) is a MADS-box transcription factor critical for central cell development in Arabidopsis embryo sacs . Key findings include:
Molecular Mechanism
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Repressor Function: AGL80 represses accessory cell-specific genes (e.g., MYB98, DD1) via interaction with TOPLESS (TPL) corepressors .
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Domain Structure: Contains an EAR motif (LNLNL) essential for transcriptional repression .
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Conservation: Phylogenetic analysis suggests its mechanism is conserved in Brassicaceae .
Experimental Evidence
Limitations and Unresolved Questions
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Antibody Specificity: The Abcam antibody targets Alpha-glucosidase, not AGL80 (transcription factor). No validated antibodies for Arabidopsis AGL80 are reported in the literature .
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Cross-Reactivity: Potential off-target binding in plant systems (e.g., to Alpha-glucosidase homologs) remains untested.
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Functional Studies: Research on AGL80 relies on transgenic reporters (e.g., AGL80pro:AGL80-GFP) rather than immunological tools .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- AGL80 functions as a transcription factor within the central cell gene regulatory network, controlling the expression of downstream genes required for central cell development and function. PMID: 16798889
- The expression pattern and mutant phenotype of AGL61 closely resemble those of AGL80, suggesting that AGL61 might function as a heterodimer with AGL80 within the central cell. PMID: 18599653
