AGO10 Antibody
Product Specs
Target Background
AGO10 is a key component of the RNA-induced silencing complex (RISC), crucial for RNA-mediated post-transcriptional gene silencing (PTGS). It binds to guide RNAs, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), directing RISC to cleave homologous mRNAs, thereby repressing gene expression. AGO10 plays a vital role in plant development, specifically in the formation and maintenance of primary and axillary shoot apical meristems. It regulates leaf adaxial identity by suppressing miR165 and miR166 microRNAs in the shoot apical meristem (SAM) and leaf primordia. Furthermore, AGO10 represses miR398, which targets CCS1 chaperone mRNAs for translational inhibition. It acts as a negative regulator of AGO1 protein levels and is essential for stem cell function and organ polarity, although it is not subject to small RNA-mediated repression itself. AGO10 also co-regulates shoot apical meristem (SAM) organization with GATA18/HAN.
Key Research Findings on AGO10 Function:
- AGO10's repression of miR165/166 inhibits shoot regeneration. (PMID: 29016889)
- AGO10 regulates shoot apical meristem size under stress conditions. (PMID: 27895202)
- HAN, expressed at boundaries, interacts with the meristem via the PNH, affecting floral organ development (JAG and BOP2) and boundary morphology (CKX3). (PMID: 26390296)
- AGO10 maintains shoot meristem stem cells by negatively regulating auxin signaling and ARF2 expression via REV. (PMID: 26358077)
- AGO1, AGO2, and AGO10 exhibit modular roles in anti-TuMV defense, with AGO2 playing a significant antiviral role in leaves. AGO5, AGO7, and AGO10 show minor effects in leaves. (PMID: 25806948)
- AGO10 maintains shoot apical meristems by binding to and quenching miR165/166. (PMID: 25801022)
- AGO10 specifies leaf adaxial identity through miR165/166 repression. (PMID: 19054365)
- AGO1 and AGO10 are crucial for the temporal regulation of floral stem cells. (PMID: 21483759)
- AGO10 specifically binds to miR166/165, determined by structural features of the miR166/165 duplex. (PMID: 21496644)
- ZWILLE maintains meristem stability non-cell-autonomously by ensuring a sufficient number of apical cells. (PMID: 14564520)
- Compromised AGO1 activity in *ago1* mutants restores siRNA and miRNA pathways for some AGO1 target genes when ZLL function is lost. (PMID: 19763164)
