AGO5 Antibody
Description
Functional Roles of AGO5
AGO5 is a catalytic RISC component critical for gene silencing and antiviral defense. Research highlights include:
Antiviral Defense Mechanisms
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AGO5 Binds Viral Small RNAs: In Nicotiana benthamiana, NbAGO5 binds virus-derived small interfering RNAs (vsiRNAs) to confer resistance against diverse viruses (e.g., potato virus X, tobacco mosaic virus) ( ).
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Viral Counter-Defense: Viruses like cucumber mosaic virus (CMV) and turnip mosaic virus (TuMV) degrade NbAGO5 via 26S proteasomes and autophagy pathways ( ).
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Jasmonic Acid Regulation: In Arabidopsis, AGO5 expression is induced by viral infection but suppressed by methyl jasmonate (MeJA), linking RNA silencing to hormonal signaling ( ).
Symbiosis and Development
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Root Nodule Formation: Phaseolus vulgaris AGO5 (PvAGO5) modulates rhizobial infection and nodule development by regulating phytohormones and cell wall biosynthesis genes ( ).
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Small RNA Sorting: AGO5 preferentially binds small RNAs with a 5′ cytosine, distinguishing its function from AGO1 (5′ U) and AGO2 (5′ A) ( ).
AGO5 in Antiviral Defense
AGO5 in Development
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Flowering Time: AGO5 interacts with miR156 to regulate Squamosa Binding Protein-Like (SPL) transcription factors in Arabidopsis ( ).
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Germline Specification: AGO5 and AGO9 coordinate transposon silencing during early germline development ( ).
Applications in Research
AGO5 antibodies enable:
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Protein Localization: Immunofluorescence reveals AGO5 accumulation in root hairs and nodule primordia during symbiosis ( ).
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Mechanistic Studies: Co-IP with vsiRNAs identifies antiviral targeting specificity ( ).
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Pathogen Interaction: Detecting AGO5 degradation by viral suppressors like CMV 2b ( ).
Technical Considerations:
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Non-specific bands may occur in Western blots due to cross-reactivity ( ).
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Optimal results require floral tissue extracts and protease inhibitors ( ).
Recent Advances (2023–2025)
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
AGO5 plays a crucial role in RNA-mediated post-transcriptional gene silencing (PTGS). As a central component of the RNA-induced silencing complex (RISC), it binds to short guide RNAs such as microRNAs (miRNAs) or small interfering RNAs (siRNAs). This complex utilizes the mature miRNA or siRNA as a guide to direct the cleavage of homologous mRNAs, thereby repressing gene expression. AGO5 exhibits a preference for associating with siRNAs ranging in size from 21 to 24 nucleotides, particularly those with a 5' terminal cytosine.
Furthermore, AGO5 is likely involved in antiviral RNA silencing. It interacts with siRNAs derived from cucumber mosaic virus (CMV). Interestingly, the turnip yellows virus (TuYV) protein P0 targets AGO5 through its F-box-like domain, leading to its degradation by the proteasome, consequently inactivating AGO5's function in RNA silencing.
- Functional and genetic role in antiviral RNA silencing. PMID: 26023161
