AGP25 Antibody
Description
Molecular Identity and Expression Profile
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Protein features: Contains a glycosylphosphatidylinositol (GPI) anchor signal, characteristic of cell-wall-localized AGPs .
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Tissue specificity:
Table 1: AGP25 Expression Patterns
| Tissue/Organ | Expression Level | Developmental Stage | Technique Used |
|---|---|---|---|
| Pistil septum | High | Pre-fertilization | Promoter-GUS fusion |
| Ovules | Moderate | Stages 11–15/16 | qPCR, immunolocalization |
| Funiculus | Moderate-High | Post-fertilization | β-Yariv reagent assay |
| Pollen grains | Absent | All stages | RNA-seq, RT-PCR |
Functional Roles in Plant Reproduction
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Nutrient signaling: AGP25 localizes to chalaza and funiculus, suggesting involvement in nutrient transfer between maternal tissues and developing embryos .
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Pollen tube guidance: Co-expressed with genes like SAH7 and WLIM2A, which regulate cytoskeletal dynamics during pollen tube growth .
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Fertilization regulation: AGP25 interacts with β-1,3-galactan chains, potentially modulating cell-wall mechanics during pollen tube reception .
Genetic and Transcriptional Regulation
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Promoter analysis: The AGP25 promoter (~3 kb upstream) drives GUS expression in ovules and funiculus, regulated by auxin-responsive elements .
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Co-expression networks: AGP25 clusters with genes involved in cell-wall remodeling (e.g., EXPANSIN A7) and nutrient transporters (e.g., SUCROSE-PROTON SYMPORTER 2) .
Comparative Analysis with Related AGPs
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Sequence homology: Shares 60–70% amino acid similarity with AGP26 and AGP27, but divergent expression patterns .
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Functional redundancy: Triple mutants (agp25/26/27) exhibit reduced seed set, suggesting partial redundancy in reproductive roles .
Research Gaps and Future Directions
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Antibody specificity: No AGP25-specific monoclonal antibody has been validated, limiting mechanistic studies.
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In vivo binding partners: Unresolved interactions with pectins or signaling receptors in the extracellular matrix.
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Biotechnological potential: AGP25 overexpression could enhance nutrient allocation in seeds, relevant for crop improvement .
