Customize AGP30 Antibody

Description

Introduction to AGP30 Antibody

AGP30 is a histidine-rich arabinogalactan protein (AGP) identified as the first non-classical AGP specifically expressed in plant roots. Unlike classical AGPs, which are broadly distributed, AGP30 plays specialized roles in root regeneration and seed development . Antibodies targeting AGP30 enable researchers to study its localization, structural properties, and functional mechanisms in plant systems.

Biological Role of AGP30

AGP30 contributes to critical developmental processes:

Root Regeneration: Essential for apical cell extension and root morphogenesis .
Seed Development: Linked to seed viability and germination efficiency .
Cell Wall Dynamics: Interacts with plasma membrane components via glycosylphosphatidylinositol (GPI) anchors, influencing cell wall permeability and signaling .

Key Studies and Outcomes

Study Focus	Methodology	Key Findings
Root Morphogenesis	βGlcYR precipitation + LM6 monoclonal antibody	AGP30 localizes to root tip regions, critical for cell elongation .
GPI Anchor Analysis	Bioinformatics + linkage analysis	AGP30 contains GPI anchor signals, enabling plasma membrane association .
Functional Knockout	Yariv reagent inhibition	AGP30 depletion disrupts root cell expansion and regeneration .

Methodological Insights

AGP30 detection relies on specialized techniques:

Monoclonal Antibodies: LM6 antibody binds (1-5)-α-L-arabinan epitopes on AGP30 glycans, enabling precise localization in root tips .
β-Glucosyl Yariv Reagent: Precipitates AGPs for biochemical analysis but may interfere with cell wall dynamics .
Immunolabeling: Requires tissue permeabilization (e.g., Triton X-100) to access epitopes at plasma membranes .

Comparative Analysis with Other AGPs

Feature	AGP30	Classical AGPs
Expression Site	Root-specific	Ubiquitous (stems, leaves, flowers)
Glycan Composition	Histidine-rich backbone + arabinan motifs	Hyp/Pro-rich backbone + type II galactans
Functional Role	Root regeneration, seed development	Cell adhesion, signaling, stress response

Future Research Directions

Structural Characterization: Resolve AGP30’s 3D conformation to elucidate glycan-protein interactions.
Agricultural Applications: Engineer AGP30 overexpression to enhance crop root resilience.
Diagnostic Potential: Explore AGP30 analogs in human health (e.g., chronic inflammation biomarkers) .

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

AGP30 antibody; At2g33790Non-classical arabinogalactan protein 30 antibody; AtAGP30 antibody

Target Names

AGP30

Uniprot No.

P93013

Target Background

Function

AGP30 Antibody targets a proteoglycan that plays a crucial role in regulating the timing of seed germination. This antibody may be involved in the abscisic acid (ABA) response pathway.

Database Links

KEGG: ath:AT2G33790

STRING: 3702.AT2G33790.1

UniGene: At.23963

Protein Families

Non-classical AGP family

Subcellular Location

Secreted, cell wall.

Tissue Specificity

Specifically expressed in root tips.

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AGP30 Antibody