At3g08860 Antibody

Overview of At3g08860

At3g08860 encodes a mitochondrial aminotransferase in Arabidopsis thaliana with dual substrate specificity:

• Primary activity: L-alanine:glyoxylate aminotransferase

• Secondary activity: β-alanine:pyruvate aminotransferase (observed in E. coli complementation assays but not confirmed in vitro)

Functional Complementation Studies

• Rescue of E. coli mutants:

  • Restores growth in β-alanine auxotroph (panD mutant) under repressible conditions .

  • Complements L-alanine auxotroph (HYE032 mutant) under inducible conditions .

Biochemical Activity

Biological Roles

• Osmoprotection: Upregulated during osmotic stress to produce β-alanine, a plant osmoprotectant .

• Photorespiration: Linked to glycine synthesis in mitochondria via glyoxylate metabolism .

Implications for Antibody Development

While no commercial or peer-reviewed At3g08860-specific antibody is documented, standard methods for polyclonal/monoclonal antibody production could be applied:

Target Antigen Design

Validation Assays

1. Western blot: Test specificity using Arabidopsis mitochondrial extracts .

2. Knockout controls: Compare wild-type and at3g08860 mutant plants .

Research Gaps

• No structural data (e.g., crystallography) exists for At3g08860 to inform epitope selection.

• Subcellular localization studies rely on predictive tools (TargetP, SUBA) without experimental validation .