AHK5 Antibody
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Description
Functional Role in Guard Cell Signaling
AHK5 mediates stomatal closure in response to multiple stimuli, including nitric oxide (NO), ethylene, darkness, and bacterial flagellin (flg22) . It operates independently of abscisic acid (ABA) signaling, positioning it in a parallel pathway for stress adaptation .
Experimental Findings
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H₂O₂ Homeostasis:
AHK5 mutants (ahk5-1, ahk5-3) show impaired stomatal closure under H₂O₂ treatment, which is rescued by complementation with wild-type AHK5 . -
Pathogen Defense:
AHK5 is essential for flagellin-induced stomatal closure but not for elf26 (another bacterial PAMP), indicating specificity in pathogen-associated molecular pattern (PAMP) responses .
Mechanistic Insights
AHK5 integrates abiotic and biotic stress signals through H₂O₂ homeostasis:
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Upstream Regulation:
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Downstream Effects:
Implications and Unanswered Questions
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
AHK5 antibody; CKI2 antibody; At5g10720 antibody; MAJ23.80Histidine kinase 5 antibody; EC 2.7.13.3 antibody; Arabidopsis histidine kinase 5 antibody; AtHK5 antibody; Protein AUTHENTIC HIS-KINASE 5 antibody; Protein CYTOKININ-INDEPENDENT 2 antibody
Target Names
AHK5
Uniprot No.
Target Background
Function
AHK5 functions as a histidine kinase and transmits stress signals to a downstream MAPK cascade. This protein undergoes ATP-dependent autophosphorylation at a conserved histidine residue within the kinase core, and a phosphoryl group is subsequently transferred to a conserved aspartate residue in the receiver domain. AHK5 acts as a negative regulator of the ETR1-dependent abscisic acid (ABA) and ethylene signaling pathway, inhibiting root elongation. It also promotes stomatal closure and regulates stomatal opening by integrating multiple signals through hydrogen peroxide (H2O2) homeostasis in guard cells, independent of ABA signaling. Additionally, AHK5 may contribute to basal defense mechanisms by closing stomata in the presence of bacterial pathogens. This protein plays a crucial role in regulating both hormone levels and ROS production in response to stress, and is essential for full immunity to bacterial pathogens and necrotrophic fungi.
Gene References Into Functions
- Data suggests the presence of a complex TCS signaling network involving AHK5, multiple AHPs and response regulators, which modulate stomatal closure in response to HO and ethylene. PMID: 22951399
- A model proposing a role for reactive oxygen species (ROS) and hormones in integrating abiotic and biotic stress responses through AHK5 has been developed. PMID: 22827948
- This is the first report demonstrating AHK5's role in regulating survival following challenge by a hemi-biotrophic bacterium and a necrotrophic fungus, as well as in the growth response to salt stress. PMID: 22256998
- Findings identify an integral signaling function for AHK5 that integrates multiple signals via H2O2 homeostasis and is independent of ABA signaling in guard cells. PMID: 18560512
Database Links
Subcellular Location
Cell membrane; Peripheral membrane protein. Cytoplasm.
Tissue Specificity
Present in light-grown but not in etiolated seedlings. Mostly expressed in roots flowers and siliques, and, to a lower extent, in stems and leaves, especially in guard cells.
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