AHL14 Antibody

AHL14 Protein Overview

AHL14 belongs to Clade B of the AHL protein family, characterized by AT-hook DNA-binding motifs and involvement in nuclear processes . Unlike Clade A AHLs (e.g., AHL15–AHL29), Clade B proteins like AHL14 have distinct C-terminal domains that influence their interaction with chromatin and other nuclear components .

Key Functions:

• Regulates chromatin remodeling and transcriptional activity .

• Implicated in plant developmental pathways, though specific mechanisms remain under investigation .

Chromatin Association and Phosphorylation

• AHL14 interacts with chromatin through its AT-hook motifs, facilitating DNA-binding and recruitment of epigenetic modifiers .

• Phosphorylation at specific serine residues (e.g., Ser313/Ser314 in AHL10, a Clade B homolog) modulates binding affinity to chromatin-associated proteins like RRP6L1, a 3’-5’ exoribonuclease .

Experimental Data:

• Pull-Down Assays: Recombinant AHL10 (Clade B) showed strong interaction with RRP6L1 in vitro, dependent on phosphorylation status .

• Mutational Analysis: Substituting serine residues (S313D/S314D) reduced RRP6L1 binding by ~40%, highlighting the importance of post-translational modifications .

Applications in Plant Biology

• Gene Expression Studies: Used to localize AHL14 in nuclear extracts, aiding in mapping protein-DNA interactions .

• Functional Knockdowns: Antibody validation supports CRISPR/Cas9-mediated gene editing experiments to study AHL14 loss-of-function phenotypes .

Comparative Analysis of AHL Antibodies

Future Directions

• Mechanistic Studies: Clarify AHL14’s role in chromatin remodeling and its crosstalk with hormonal signaling.

• Cross-Species Validation: Test antibody reactivity in related plant species (e.g., Brassica napus) .