AHP4 Antibody

Definition and Biological Role

AHP4 antibody targets the histidine phosphotransfer protein 4 (AHP4) in Arabidopsis thaliana, which mediates signal transduction from sensor histidine kinases to nuclear response regulators . This protein is critical for:

• Magnesium-dependent phosphorelay in plant stress responses .

• Secondary cell wall formation in anther endothecium .

• Internal clock regulation and drought stress adaptation .

Antibody Development and Validation

• Source: Generated via hybridoma technology at the Central European Institute of Technology (CEITEC), Czech Republic .

• Validation:

  • Specificity confirmed for AHP4 isoforms using Western blot (1:10,000 dilution) .

  • Knockout validation in plant models to ensure no cross-reactivity with related proteins .

Applications in Research

The AHP4 antibody is utilized in:

Key Research Findings

• Stress Response: AHP4 is upregulated during drought, facilitating signal transfer to regulate stress adaptation .

• Developmental Role: Critical for secondary cell wall thickening in anthers, impacting plant reproduction .

• Signaling Specificity: The antibody distinguishes AHP4 from other AHP family members (e.g., AHP2, AHP3) .

Comparison with Related Antibodies

The AHP4 antibody is often used alongside other AHP-targeting antibodies (e.g., AHP2@A4, AHP3@1A10) to map signaling networks . Unlike broader plant antibodies, it provides isoform-specific insights.

Limitations and Considerations

• Species Restriction: Exclusively validated in Arabidopsis thaliana .

• Storage Sensitivity: Requires stringent -80°C storage to maintain stability .