DPBF3 Antibody

DPBF3 Overview

DPBF3 (ABA-Responsive Element Binding Protein 3, AT3G56850) is a bZIP transcription factor in Arabidopsis that regulates ABA-dependent gene expression, particularly during seed maturation and stress responses . Key features include:

Mechanistic Role of DPBF3

• Regulation of ADF5: DPBF3 binds directly to the ADF5 promoter via ACG motifs, activating its transcription in response to ABA. This promotes actin filament reorganization, enabling stomatal closure under drought stress .

• Interaction with ABA Signaling: DPBF3 is part of a network involving ABF/AREB transcription factors, though it does not redundantly function with ABF1–ABF4 in stomatal movement .

Antibody Development Considerations

While no DPBF3-specific antibody is documented in the provided sources, general principles for antibody design (e.g., epitope selection, validation) can be inferred from adjacent research:

Key Parameters for Antibody Design

Research Gaps and Opportunities

1. Antibody Development: No commercial or experimental DPBF3 antibodies are reported. Prioritize epitopes in the bZIP domain or phosphorylation sites for functional studies.

2. Functional Studies: Further investigate DPBF3’s role in ABA-independent pathways or cross-talk with other stress regulators.

3. Structural Analysis: Resolve DPBF3’s 3D structure to guide targeted mutagenesis or inhibitor design.