ABF1 Antibody

What is ABF1 Antibody?

ABF1 antibodies are laboratory-generated proteins designed to bind specifically to ABF1, a multifunctional factor with distinct roles depending on the organism:

• Yeast ABF1: A DNA-binding protein in Saccharomyces cerevisiae involved in replication, transcriptional regulation, and global genome nucleotide excision repair (GG-NER) .

• Plant ABF1: An abscisic acid (ABA)-responsive transcription factor in Arabidopsis thaliana mediating stress responses .

• Human ABF1 (MSC): A basic helix-loop-helix transcriptional repressor regulating B-cell differentiation and plasma cell formation .

These antibodies are pivotal for detecting ABF1 in experimental workflows such as Western blot (WB), ELISA, and chromatin immunoprecipitation (ChIP).

Types and Targets of ABF1 Antibodies

ABF1 antibodies vary by target species and applications:

Yeast ABF1

• DNA Repair: ABF1 facilitates GG-NER by recruiting repair complexes (e.g., Rad7-Rad16) to damaged DNA sites. Mutations in ABF1-binding sites impair repair efficiency .

• Transcriptional Regulation: Binds promoters of ~100+ genes, influencing metabolic pathways and cell cycle progression. ChIP-Chip assays identified 434 direct target loci .

• Replication: Essential for origin activation at ARS1 and silencing at mating-type loci .

Plant ABF1

• Regulates ABA-responsive genes under drought and osmotic stress. The antibody A22789 detects ABF1 at ~39 kDa in Arabidopsis tissues .

Human ABF1 (MSC)

• Immune Regulation: Suppresses plasma cell differentiation by repressing PRDM1 (Blimp1) and promoting memory B-cell formation. Knockdown increases antibody-secreting cells .

• Cancer: Overexpressed in Hodgkin’s lymphoma, where it blocks terminal B-cell differentiation .

Applications in Research and Therapy

• Mechanistic Studies: ABF1 antibodies validate protein-DNA interactions via EMSA and ChIP .

• Agricultural Research: Monitoring ABA-responsive genes in transgenic plants under stress .

• Immunotherapy: Targeting ABF1 in B-cell malignancies to restore differentiation pathways .

Key Challenges and Innovations

• Cross-Reactivity: Antibodies for yeast ABF1 may not recognize plant or human homologs due to low sequence conservation.

• Therapeutic Potential: Human ABF1 inhibitors are under exploration for autoimmune diseases and lymphoma .