ABF3 Antibody

Role in ABA Signaling

• ABF3 is phosphorylated by the ABA-activated kinase OST1 on residue T451, creating a 14-3-3 binding motif critical for its stabilization and turnover .

• ABF3 interacts with NAC072 to cooperatively regulate ABA-responsive genes like RD29A but antagonistically modulates RD29B expression .

• Degradation of ABF3 is mediated by the E3 ubiquitin ligase KEEP ON GOING (KEG), which directly ubiquitylates ABF3 in vitro and regulates its stability in vivo .

Nitrate Response in Roots

• ABF3 is a hub transcription factor in the endodermis cell layer, controlling >50% of nitrate-responsive genes.

• abf3 mutants exhibit reduced lateral root density under nitrate treatment, linking ABF3 to root architecture modulation .

Table: ABF3-Associated Pathways and Interactions

ABF3 Antibody Applications

The ABF3 antibody has been instrumental in:

• Detecting ABF3 protein levels in nitrate-treated root tissues .

• Validating ABF3-KEG interaction studies via Western blot .

• Confirming ABF3 phosphorylation status in ABA-treated guard cells .

Research Gaps and Future Directions

• Spatiotemporal Regulation: ABF3 exhibits cell type-specific posttranscriptional regulation, with protein localization differing from mRNA expression patterns .

• Cross-Species Relevance: While ABF3 is well-studied in Arabidopsis, its homologs in crops remain underexplored.